中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2014年
14期
890-894
,共5页
徐冬%吕晓伟%毕作木%任国华%王鲁群
徐鼕%呂曉偉%畢作木%任國華%王魯群
서동%려효위%필작목%임국화%왕로군
c-maf基因%多发性骨髓瘤%RNA干扰%增殖%侵袭%凋亡
c-maf基因%多髮性骨髓瘤%RNA榦擾%增殖%侵襲%凋亡
c-maf기인%다발성골수류%RNA간우%증식%침습%조망
c-maf gene%multiple myeloma%RNAi%proliferation%invasion%apoptosis
目的:探讨c-maf基因对多发性骨髓瘤细胞增殖、侵袭力的影响。方法:脂质体法用c-maf siRNA转染多发性骨髓瘤细胞系RPMI8226,RT-PCR技术检测c-maf基因的表达,MTT实验和Transwell小室分别检测多发性骨髓瘤细胞增殖活性和侵袭力的改变,流式细胞术检测细胞周期,Western blots检测相关蛋白的表达情况,并检测Caspase的活性。结果:c-maf siRNA有效地转染入细胞并抑制了c-maf基因的表达。转染c-maf siRNA细胞的增殖活性及体外侵袭力均显著下降(P<0.05),细胞周期阻滞在G2/M期,survivin、MMP-2、MMP-9、ARK5、cyclin B1蛋白水平明显低于对照组(P<0.05),Caspase-3/7蛋白活性高于对照组(P<
目的:探討c-maf基因對多髮性骨髓瘤細胞增殖、侵襲力的影響。方法:脂質體法用c-maf siRNA轉染多髮性骨髓瘤細胞繫RPMI8226,RT-PCR技術檢測c-maf基因的錶達,MTT實驗和Transwell小室分彆檢測多髮性骨髓瘤細胞增殖活性和侵襲力的改變,流式細胞術檢測細胞週期,Western blots檢測相關蛋白的錶達情況,併檢測Caspase的活性。結果:c-maf siRNA有效地轉染入細胞併抑製瞭c-maf基因的錶達。轉染c-maf siRNA細胞的增殖活性及體外侵襲力均顯著下降(P<0.05),細胞週期阻滯在G2/M期,survivin、MMP-2、MMP-9、ARK5、cyclin B1蛋白水平明顯低于對照組(P<0.05),Caspase-3/7蛋白活性高于對照組(P<
목적:탐토c-maf기인대다발성골수류세포증식、침습력적영향。방법:지질체법용c-maf siRNA전염다발성골수류세포계RPMI8226,RT-PCR기술검측c-maf기인적표체,MTT실험화Transwell소실분별검측다발성골수류세포증식활성화침습력적개변,류식세포술검측세포주기,Western blots검측상관단백적표체정황,병검측Caspase적활성。결과:c-maf siRNA유효지전염입세포병억제료c-maf기인적표체。전염c-maf siRNA세포적증식활성급체외침습력균현저하강(P<0.05),세포주기조체재G2/M기,survivin、MMP-2、MMP-9、ARK5、cyclin B1단백수평명현저우대조조(P<0.05),Caspase-3/7단백활성고우대조조(P<
Objective:To investigate the effect of c-maf gene on the MM cells' proliferation and invasion activity. Methods:Lipo-fectin Reagent was used to transfect c-maf siRNA into multiple myeloma cell of RPMI8226. The mRNA expression level of c-maf was detected by RT-PCR.Cell growth curve was measured by MTT assay. Transwell chamber test was used to measure MM cells' in vitro in-vasion activity. The cell cycle distribution were assessed by flow cytomentry. The protein expression levels of survivin,MMP-2, MMP-9, ARK5 and cyclin B1 were detected by Western blot. We also detected the activity of Caspase-3/7. Results:The c-maf siRNA was effectively transfected into cells and the mRNA expression of the c-maf gene was inhibited.MTT test and Transwell chamber test showed that the proliferation and in vitro invasion activity of transfected cells were significantly lower than those of other two groups (P<0.05). Cell cycle of c-maf siRNA transfected group cells was arrested in G2/M phase. The expression levels of survivin,MMP-2, MMP-9,ARK5,cyclin B1 and the activity of Caspase-3/7 between c-maf siRNA transfected group and the other two groups were sta-tistically different (P<0.05). Conclusion:c-maf gene by c-maf siRNA can remarkably inhibit proliferation and invasion of multiple my-eloma cell lines of RPMI8226. C-maf gene may be used as the target for multiple myeloma gene therapy.