CAJ | 학술논문

背景：玻璃离子水门汀充填的儿童牙体仍易发生继发龋现象，与充填材料表面牙菌斑内复杂微生物群落具有密切关系，而传统微生物学方法无法获知牙菌斑微生物的重要信息。目的：利用先进的现代分子生态学技术解析不同龋敏感儿童玻璃离子水门汀表面牙菌斑内微生物群落结构与重要致龋微生物的数量水平。方法：选择3-5岁儿童24名，按乳牙龋失补牙面指数不同分为无龋组、中龋组和高龋组，每组8名。采集各组儿童全口牙面菌斑，利用变性梯度凝胶电泳进行微生物群落多样性分析与微生物种群鉴定，利用荧光原位杂交考察致龋微生物Streptococcus spp.的数量分布，利用实时定量PCR考察重要致龋菌Streptococcus mutans占总菌的相对数量。结果与结论：无龋组牙菌斑内微生物群落的多样性显著高于中龋组和高龋组(P <0.05)，中龋组和高龋组中大量富集的某些微生物可能在龋病发展过程中起重要作用。3组样本共检出16个微生物菌属，Streptococcus spp.和Actinomycesspp.可能是高龋组中重要的致龋微生物。Streptococcus spp.和Streptococcus mutans在高龋组中所占比例显著高于无龋组和中龋组(P <0.01)。综合来看，研究采用的分子生态学技术可以较好反映牙菌斑内与致龋过程密切相关的复杂微生物群落。
배경：파리리자수문정충전적인동아체잉역발생계발우현상，여충전재료표면아균반내복잡미생물군락구유밀절관계，이전통미생물학방법무법획지아균반미생물적중요신식。목적：이용선진적현대분자생태학기술해석불동우민감인동파리리자수문정표면아균반내미생물군락결구여중요치우미생물적수량수평。방법：선택3-5세인동24명，안유아우실보아면지수불동분위무우조、중우조화고우조，매조8명。채집각조인동전구아면균반，이용변성제도응효전영진행미생물군락다양성분석여미생물충군감정，이용형광원위잡교고찰치우미생물Streptococcus spp.적수량분포，이용실시정량PCR고찰중요치우균Streptococcus mutans점총균적상대수량。결과여결론：무우조아균반내미생물군락적다양성현저고우중우조화고우조(P <0.05)，중우조화고우조중대량부집적모사미생물가능재우병발전과정중기중요작용。3조양본공검출16개미생물균속，Streptococcus spp.화Actinomycesspp.가능시고우조중중요적치우미생물。Streptococcus spp.화Streptococcus mutans재고우조중소점비례현저고우무우조화중우조(P <0.01)。종합래간，연구채용적분자생태학기술가이교호반영아균반내여치우과정밀절상관적복잡미생물군락。
BACKGROUND:Children teeth filed with glass ionomer cement are stil susceptible with secondary caries, which is in close relationship with complex microbial community in dental plaque on the surface of glass ionomer cement. Traditional microbial methods are incapable of getting important information towards dental plaque microbes. OBJECTIVE:To analyze microbial community structure and numerical level of caries-induced microbes in dental plaque on the surface of glass ionomer cement for different caries-susceptible children. METHODS:Twenty-four children (age: 3-5 years) were divided into the caries-free, caries-positive, and caries-active children groups by the decayed, missing and filed index. With eight individuals in each group, their dental plaques were sampled for microbial community analysis. Denaturing gradient gel electrophoresis was employed to make clear the microbial community diversity and species identity in dental plaque of the caries-free, caries-positive, and caries-active children groups. Fluorescentin situ hybridization was used to investigate the numerical level of the caries-induced microbeStreptococcus spp. Quantitative PCR was carried out to analyze relative quantity ofStreptococcusmutans in total bacteria. RESULTS AND CONCLUSION:Compared with the caries-positive and caries-active children groups, microbial community diversity among samples was significantly higher in the caries-free group. Microbes abound in the caries-positive and caries-active groups might act important roles in the development of caries.Streptococcus spp. and Actinomyces spp. might be important caries-induced microbes in the caries-active group. The ratios ofStreptococcus spp. andStreptococcus mutans in total bacteria were significantly higher in the caries-free group than those in the caries-free and caries-positive groups. In summary, molecular ecology technologies can wel reflect caries-related complex microbial community in dental plaque.