CAJ | 학술논문

背景：纤维蛋白是一种天然的可生物降解、组织相容性好的高分子材料，是一种能促进细胞和外源性生长因子释放的载体，其中血纤维蛋白稳定因子ⅩⅢ已证明有利于未分化的间充质干细胞在高度交联的凝胶支架内迁移，并且促进这些细胞的增殖与分化能力。目的：观察大鼠间充质干细胞在纤维蛋白凝胶内的行为。方法：无菌条件下分离大鼠胎肢细胞获得间充质干细胞，取第3代细胞分别接种于0，5，10，20 g/L纤维蛋白凝胶内，用倒置相差显微镜和激光扫描共聚焦显微镜分析细胞在凝胶内的形态学变化；酶标仪和Von Kossa染色分析碱性磷酸酶活性和钙盐沉积。结果与结论：5 g/L低浓度纤维蛋白凝胶有利于细胞形态的发生，20 g/L高浓度凝胶有利于细胞的成骨分化。20 g/L纤维蛋白凝胶碱性磷酸酶活性高于对照组，10和20 g/L浓度纤维蛋白凝胶矿化结节出现在21至28 d，而对照组无矿化结节出现。提示大鼠间充质干细胞的形态与成骨分化依赖于纤维蛋白凝胶浓度，提示纤维蛋白凝胶有助于间充质干细胞的成骨分化。
배경：섬유단백시일충천연적가생물강해、조직상용성호적고분자재료，시일충능촉진세포화외원성생장인자석방적재체，기중혈섬유단백은정인자ⅩⅢ이증명유리우미분화적간충질간세포재고도교련적응효지가내천이，병차촉진저사세포적증식여분화능력。목적：관찰대서간충질간세포재섬유단백응효내적행위。방법：무균조건하분리대서태지세포획득간충질간세포，취제3대세포분별접충우0，5，10，20 g/L섬유단백응효내，용도치상차현미경화격광소묘공취초현미경분석세포재응효내적형태학변화；매표의화Von Kossa염색분석감성린산매활성화개염침적。결과여결론：5 g/L저농도섬유단백응효유리우세포형태적발생，20 g/L고농도응효유리우세포적성골분화。20 g/L섬유단백응효감성린산매활성고우대조조，10화20 g/L농도섬유단백응효광화결절출현재21지28 d，이대조조무광화결절출현。제시대서간충질간세포적형태여성골분화의뢰우섬유단백응효농도，제시섬유단백응효유조우간충질간세포적성골분화。
BACKGROUND: Fibrin is a kind of high polymer materials with biodegradation and good histocompatibility, and is a vector that can promote celland exogenous growth factor release. Fibrin stabilizing factor XIII has been verified to contribute to the migration of undifferentiated mesenchymal stem cels in gel scaffold with high crosslinking, and promote cellproliferation and differentiation. OBJECTIVE:To observe rat mesenchymal stem cellbehavior in a fibrin gel. METHODS:The rat fetal limbs cels was separated under the aseptic condition. The passage 3 cels were seeded in 0, 5, 10 and 20 g/L fibrin gel. cellmorphology was observed by inverted phase microscope and laser scanning confocal microscopy. Alkaline phosphatase activity and calcium deposition were measured respectively using a microplate reader and von Kossa staining. RESULTS AND CONCLUSION: 5 g/L fibrin gel contributed to cellmorphological changes, and 20 g/L fibrin gel contributed to osteogenic differentiation. Compared with the control group, alkaline phosphatase activity was higher in the formulations containing a 20 g/L fibrinogen concentration. Smal mineralization nodules were observed at 21 and 28 days in a formulation containing both 10 and 20 g/L fibrinogen concentration, but no mineralization was detected in the control group. These results indicate that morphology and osteogenic differentiation of rat mesenchymal stem cels depended on the fibrinogen concentration, suggesting that fibrin gel is conducive to osteogenic differentiation of mesenchymal stem cels.