CAJ | 학술논문

背景：课题组和青岛大学高分子材料研究所合作研制的海藻纤维生物膜，具有优良的生物相容性，常被用作制备各种复合材料。目的：观察海藻纤维膜片包绕覆盖神经断端吻合口对大鼠坐骨神经损伤后再生的影响。方法：切断36只雄性Wistar大鼠右侧坐骨神经，随机分组：对照组行神经外膜端端吻合；实验组行神经外膜端端缝合，将海藻纤维膜片包绕并覆盖神经吻合口远近端各约0.5 cm，形成封闭再生室。术后观察海藻纤维膜片降解吸收规律及缝合处粘连情况，组织学切片行苏木精-伊红染色、锇酸染色、白细胞介素2及白细胞介素4免疫组织化学染色。结果与结论：术后4-6周，实验组海藻纤维膜片逐渐被降解吸收，与周围组织粘连较少，炎性细胞浸润程度较轻，纤维组织增生较少。两组术后1，7，14 d的白细胞介素2及白细胞介素4含量比较差异无显著性意义。实验组术后6周再生神经纤维分布规则且大小较为均一，其神经纤维数量、轴突大小及髓鞘厚度等指标均显著优于对照组(P <0.05)。表明海藻纤维膜片具有良好的生物降解性和组织相容性，其包绕覆盖坐骨神经形成的神经再生密闭室可促进大鼠损伤坐骨神经再生。
배경：과제조화청도대학고분자재료연구소합작연제적해조섬유생물막，구유우량적생물상용성，상피용작제비각충복합재료。목적：관찰해조섬유막편포요복개신경단단문합구대대서좌골신경손상후재생적영향。방법：절단36지웅성Wistar대서우측좌골신경，수궤분조：대조조행신경외막단단문합；실험조행신경외막단단봉합，장해조섬유막편포요병복개신경문합구원근단각약0.5 cm，형성봉폐재생실。술후관찰해조섬유막편강해흡수규률급봉합처점련정황，조직학절편행소목정-이홍염색、철산염색、백세포개소2급백세포개소4면역조직화학염색。결과여결론：술후4-6주，실험조해조섬유막편축점피강해흡수，여주위조직점련교소，염성세포침윤정도교경，섬유조직증생교소。량조술후1，7，14 d적백세포개소2급백세포개소4함량비교차이무현저성의의。실험조술후6주재생신경섬유분포규칙차대소교위균일，기신경섬유수량、축돌대소급수초후도등지표균현저우우대조조(P <0.05)。표명해조섬유막편구유량호적생물강해성화조직상용성，기포요복개좌골신경형성적신경재생밀폐실가촉진대서손상좌골신경재생。
BACKGROUND:The sodium alginate film, jointly developed by our research team and the Institute of Polymer Material of Qingdao University, has good biocompatibility and is often used for the preparation of a variety of composite materials. OBJECTIVE: To observe the effect of sodium alginate film wrapping and covering nerve anastomotic stoma on the regeneration of injured sciatic nerve in rats. METHODS: A total of 36 male Wistar rats were randomly divided into two groups after the right sciatic nerve was cut. Control group: The nerve stump was sutured through epineurial end-to-end anastomosis; Experimental group: The nerve stump was sutured through epineurial end-to-end anastomosis, and wrapped with sodium alginate film, forming a regenerative environment for sciatic nerve regeneration. The degradation and absorption of sodium alginate film, as wel as the adhesion at suture site were observed post-operation. Interleukin-2 and interleukin-4 positive expression was detected with immunohistochemistry method. The histological sections were also detected with hematoxylin-eosin staining and osmium tetroxide staining. RESULTS AND CONCLUSION:At 4-6 weeks post-operation, sodium alginate film was mostly degraded and absorbed in the experimental group. The experimental group rats had less local adhesion, slighter infiltration of inflammatory cels, and fewer fiber tissue hyperplasia than control group rats. The contents of interleukin-2 and interleukin-4 were almost the same between the control and experimental groups at 1, 7, 14 days post-operation. At 6 weeks, the regenerated nerve fibers were distributed evenly with uniform shape, the number of nerve fibers, axons and myelin sheath were significantly better than the control group (P < 0.05). Sodium alginate film has good biological degradability and histocompatibility and plays an important role in promoting sciatic nerve regeneration.