CAJ | 학술논문

针对临床上难治的管状骨长段骨缺损的组织工程化治疗的研究多集中在支架材料形状、形态、尺寸的设计上，但都没有直接检测接种的细胞数量，尤其是有活力细胞多少的检测，目前只能通过间接指标比如电镜扫描和成骨情况等来推断接种细胞的有效程度。作者自制瓦楞状组织工程骨支架和其他3种形状的支架，并通过4种不同形状支架负载种子细胞数量的研究和该瓦楞状组织工程骨支架体内成骨的研究论述自制支架瓦楞状凹槽的优势及特点。
침대림상상난치적관상골장단골결손적조직공정화치료적연구다집중재지가재료형상、형태、척촌적설계상，단도몰유직접검측접충적세포수량，우기시유활력세포다소적검측，목전지능통과간접지표비여전경소묘화성골정황등래추단접충세포적유효정도。작자자제와릉상조직공정골지가화기타3충형상적지가，병통과4충불동형상지가부재충자세포수량적연구화해와릉상조직공정골지가체내성골적연구논술자제지가와릉상요조적우세급특점。
BACKGROUND:The effects of engineered bone scaffold containing seeding cels with different shapes to repair bone defect are varied, while the loaded cellquantity is the important factor influencing the curative effect, but which is rarely reported. OBJECTIVE:By preparing self-made corrugated tissue-engineered bone scaffold and other three forms of bone tissue engineering scaffolds, to study the quantity of loaded cels on different scaffolds and osteogenesis of corrugated tissue-engineered bone scaffold so as to discuss the advantages and features of self-made corrugated tissue-engineered bone scaffold. METHODS: (1) Experimentin vitro: There were four kinds of scaffolds with the same volume and samples: calcium phosphate cement (CPC) corrugated surface scaffold group, smooth surface scaffold group, cylindrical scaffold group and porous cylindrical scaffold with holow tubes group, in which the latter three groups are control ones. A certain volume with same density of rabbit bone marrow mesenchymal stem cels (BMSCs) suspension after osteogenesis induction was seeded onto the scaffolds. After incubation, culture, digestion and colection, cellquantity was counted, absorbance value was finaly detected and cellactivity was proofed by alkaline phosphatase and alizarin red staining. (2) Experimentin vivo: New Zealand rabbits were randomly and equaly divided into recombinant human bone morphogenetic protein-2 (rhBMP-2)/CPC/BMSCs corrugated scaffold group, pure CPC corrugated scaffold group and cancelous bone implant group. Three kinds of scaffold implants with the same volume were inserted into the area between rabbit’s L5, 6 transverse processes bilateraly. At 4, 8, 12 weeks postoperatively, gross and histological observation was performed. RESULTS AND CONCLUSION:(1)Experimentin vitro: The drip of cellsuspension steadily stayed on the surface of corrugated scaffold because of corrugated shape groove and the surface tension of the liquid. The amount of cels per sample digested down from the CPC corrugated surface scaffold was significantly more than that from the other three groups (P < 0.05), while the absorbance values did not differ (P > 0.05). (2) Experimentin vivo: At each time point the osteogenesis quantity of rhBMP-2/CPC/BMSCs corrugated scaffold group was more than that of the pure CPC corrugated scaffold group (P < 0.05), while there was no difference from the cancelous bone implant group (P > 0.05). These findings indicate that the characteristics of the self-made corrugated engineered bone scaffold are beneficial to seed cellloading, which supports a large number of osteogenesis and provides feasibility to promote the healing of segmental bone defects.