世界最新医学信息文摘（电子版）
世界最新醫學信息文摘（電子版）
세계최신의학신식문적（전자판）
World Latest Medicine Information
2014年
21期
11-11,13
,共2页
高原地区%Ⅱ型糖尿病%对氧磷酯酶 1.2%s311c 基因多态性
高原地區%Ⅱ型糖尿病%對氧燐酯酶 1.2%s311c 基因多態性
고원지구%Ⅱ형당뇨병%대양린지매 1.2%s311c 기인다태성
plateau area%diabetes%paraoxonase 1.2%s311c gene polymorphism s311c Polymorphism
目的：测定对氧磷酶（Pon2）基因多态性在老年糖尿病人群中的分布，分析不同基因型与糖尿病血管并发症的关系。方法183例患者分为糖尿病（t2dM）合并大血管病变组、糖尿病合并微血管病变组、单纯糖尿病组及对照组。应用多聚酶链反应-限制性片断长度多态性( Pcr-rflP)结合测序法对 Pon2 s311c 多态性进行基因分型,测定两组人群的基因型频率和等位基因。结果 dM 大血管病变组基因频率 ss 为24(68.6%)，cs 为10(28.6%)、cc 为1(2.9%)，dM 微血管病变组基因频率 ss 为14(56.0%)，cs 为9(36.0%)、cc 为2(8.0%)，单纯 dM 组 ss 为5(27.8%)，cs 为7(38.9%、cc 为6(33.3%)；对照组 ss 为59(67.1%)，cs 为25(28.4%)、cc 为4(4.6%)。4组之间，基因分布频率差异有统计学意义（P ＜0.05）。4组中等位基因 s 的频率分别为82.9%、76.0%、44.5%和80.7%，等位基因 c 的频率分别为17.1%、24.0%、55.5%和19.3%，等位基因的差异有统计学意义（P ＜0.05）。结论 Pon2 cys311ser 等位基因可能是 t2dM及大血管并发症的易感遗传标志。
目的：測定對氧燐酶（Pon2）基因多態性在老年糖尿病人群中的分佈，分析不同基因型與糖尿病血管併髮癥的關繫。方法183例患者分為糖尿病（t2dM）閤併大血管病變組、糖尿病閤併微血管病變組、單純糖尿病組及對照組。應用多聚酶鏈反應-限製性片斷長度多態性( Pcr-rflP)結閤測序法對 Pon2 s311c 多態性進行基因分型,測定兩組人群的基因型頻率和等位基因。結果 dM 大血管病變組基因頻率 ss 為24(68.6%)，cs 為10(28.6%)、cc 為1(2.9%)，dM 微血管病變組基因頻率 ss 為14(56.0%)，cs 為9(36.0%)、cc 為2(8.0%)，單純 dM 組 ss 為5(27.8%)，cs 為7(38.9%、cc 為6(33.3%)；對照組 ss 為59(67.1%)，cs 為25(28.4%)、cc 為4(4.6%)。4組之間，基因分佈頻率差異有統計學意義（P ＜0.05）。4組中等位基因 s 的頻率分彆為82.9%、76.0%、44.5%和80.7%，等位基因 c 的頻率分彆為17.1%、24.0%、55.5%和19.3%，等位基因的差異有統計學意義（P ＜0.05）。結論 Pon2 cys311ser 等位基因可能是 t2dM及大血管併髮癥的易感遺傳標誌。
목적：측정대양린매（Pon2）기인다태성재노년당뇨병인군중적분포，분석불동기인형여당뇨병혈관병발증적관계。방법183례환자분위당뇨병（t2dM）합병대혈관병변조、당뇨병합병미혈관병변조、단순당뇨병조급대조조。응용다취매련반응-한제성편단장도다태성( Pcr-rflP)결합측서법대 Pon2 s311c 다태성진행기인분형,측정량조인군적기인형빈솔화등위기인。결과 dM 대혈관병변조기인빈솔 ss 위24(68.6%)，cs 위10(28.6%)、cc 위1(2.9%)，dM 미혈관병변조기인빈솔 ss 위14(56.0%)，cs 위9(36.0%)、cc 위2(8.0%)，단순 dM 조 ss 위5(27.8%)，cs 위7(38.9%、cc 위6(33.3%)；대조조 ss 위59(67.1%)，cs 위25(28.4%)、cc 위4(4.6%)。4조지간，기인분포빈솔차이유통계학의의（P ＜0.05）。4조중등위기인 s 적빈솔분별위82.9%、76.0%、44.5%화80.7%，등위기인 c 적빈솔분별위17.1%、24.0%、55.5%화19.3%，등위기인적차이유통계학의의（P ＜0.05）。결론 Pon2 cys311ser 등위기인가능시 t2dM급대혈관병발증적역감유전표지。
Objective to determine the paraoxonase (Pon2) gene polymorphism in the distribution of elderly diabetic population and analyze the relationship between different genotype and vascular complications of diabetes. Methods 183 patients were divided into diabetes mellitus (t2dM) complicated with macroangiopathy group, diabetic microangiopathy group, simple diabetic group and control group. the genotype frequency and allele in two groups were determined after the Pon2 s311c polymorphism was genotyped by application of polymerase chain reaction restriction fragment length polymorphism (Pcr-rflP) combined with sequencing. Results ss gene frequency in dM macroangiopathy group was 24 (68.6%), cs 10 (28.6%), cc 1 (2.9%) and that in dM microangiopathy group was 14 (56%), cs 9 (36%), cc 2 (8%), dM group (ss 5 27.8%), cs 7 (38.9%, cc was 6 (33.3%), the control group was 59 (ss 67.1%), cs 25 (28.4%), cc 4 (4.6%). among these 4 groups, the frequency distribution of genetic differences were statistically significant (P < 0.05). the frequency of allele s in the 4 groups were 82.9%, 76%, 44.5% and 80.7%. the frequencies of c allele were 17.1%, 24%, 55.5% and 19.3%, the differences were statistically significant(P < 0.05).Conclusion Pon2 cys311ser allele may be a susceptible genetic markers in t2dM and macrovascular complications.
