CAJ | 학술논문

采用PCR直接测序法对伞形科( Apiaceae)前胡族( Trib. Peucedaneae)种类川明参( Chuanminshen violaceum Sheh et Shan)、泰山前胡〔Peucedanum wawrae ( H. Wolff) Su〕和华中前胡( P. medicum Dunn)以及美味芹族( Trib. Smyrnieae)种类明党参(Changium smyrnioides H. Wolff)、宝兴棱子芹(Pleurospermum davidii Franch.)、丽江棱子芹( P. foetens Franch.)和鸡冠棱子芹( P. cristatum de Boiss.)的叶绿体基因组psbA-trnH片段进行了扩增测序,获得的序列已提交至GenBank,登录号为KF557756-KF557762。结合引自GenBank的前胡族阿魏属( Ferula Linn.)1种、大瓣芹属( Semenovia Regel et Herder)1种、当归属( Angelica Linn.)2种和美味芹族的舟瓣芹属( Sinolimprichtia H. Wolff)1种、羌活属( Notopterygium de Boiss.)1种、瘤果芹属( Trachydium Lindl.)1种以及针果芹族( Trib. Scandicineae)刺果芹〔Turgenia latifolia ( Linn.) Hoffm.〕的psbA-trnH片段序列,对各种类的psbA-trnH片段信息进行分析；并以刺果芹为外类群构建了MP、ML和BI系统发育树。结果表明：川明参和明党参的psbA-trnH片段长度均为258 bp、GC含量均为23％,而其他种类的psbA-trnH片段长度为228~405 bp、GC含量为26％~35％；排序后psbA-trnH 序列总长度为553 bp(包括空位),其中变异位点237个、信息位点178个。川明参与明党参间的相对遗传距离最小(仅为0.02),而川明参与其他种类间的相对遗传距离为0.10~1.34,且总体上川明参与美味芹族种类的相对遗传距离较小,表明川明参与明党参及美味芹族种类的亲缘关系较近。在3类系统树上,川明参与明党参均聚在一起,并与美味芹族的属种聚为一大支,而远离由前胡族属种构成的另一大支。结合外部形态和果实解剖结构特征,建议将川明参属( Chuanminshen Sheh et Shan )从前胡族中分出并置于美味芹族中,与明党参属( Changium H. Wolff)为姐妹类群。
채용PCR직접측서법대산형과( Apiaceae)전호족( Trib. Peucedaneae)충류천명삼( Chuanminshen violaceum Sheh et Shan)、태산전호〔Peucedanum wawrae ( H. Wolff) Su〕화화중전호( P. medicum Dunn)이급미미근족( Trib. Smyrnieae)충류명당삼(Changium smyrnioides H. Wolff)、보흥릉자근(Pleurospermum davidii Franch.)、려강릉자근( P. foetens Franch.)화계관릉자근( P. cristatum de Boiss.)적협록체기인조psbA-trnH편단진행료확증측서,획득적서렬이제교지GenBank,등록호위KF557756-KF557762。결합인자GenBank적전호족아위속( Ferula Linn.)1충、대판근속( Semenovia Regel et Herder)1충、당귀속( Angelica Linn.)2충화미미근족적주판근속( Sinolimprichtia H. Wolff)1충、강활속( Notopterygium de Boiss.)1충、류과근속( Trachydium Lindl.)1충이급침과근족( Trib. Scandicineae)자과근〔Turgenia latifolia ( Linn.) Hoffm.〕적psbA-trnH편단서렬,대각충류적psbA-trnH편단신식진행분석；병이자과근위외류군구건료MP、ML화BI계통발육수。결과표명：천명삼화명당삼적psbA-trnH편단장도균위258 bp、GC함량균위23％,이기타충류적psbA-trnH편단장도위228~405 bp、GC함량위26％~35％；배서후psbA-trnH 서렬총장도위553 bp(포괄공위),기중변이위점237개、신식위점178개。천명삼여명당삼간적상대유전거리최소(부위0.02),이천명삼여기타충류간적상대유전거리위0.10~1.34,차총체상천명삼여미미근족충류적상대유전거리교소,표명천명삼여명당삼급미미근족충류적친연관계교근。재3류계통수상,천명삼여명당삼균취재일기,병여미미근족적속충취위일대지,이원리유전호족속충구성적령일대지。결합외부형태화과실해부결구특정,건의장천명삼속( Chuanminshen Sheh et Shan )종전호족중분출병치우미미근족중,여명당삼속( Changium H. Wolff)위저매류군。
By means of PCR amplification and sequencing methods, cpDNA psbA-trnH fragment from Chuanminshen violaceum Sheh et Shan, Peucedanum wawrae ( H. Wolff) Su and P. medicum Dunn of Trib. Peucedaneae, Changium smyrnioides H. Wolff, Pleurospermum davidii Franch., P. foetens Franch. and P. cristatum de Boiss. of Trib. Smyrnieae in Apiaceae were amplified and sequenced, sequences obtained were submitted to GenBank and their accession numbers were KF557756-KF557762 . In combination of psbA-trnH fragment sequences, induced from GenBank, of one species in Ferula Linn., one species in Semenovia Regel et Herder and two species in Angelica Linn. of Trib. Peucedaneae, one species in Sinolimprichtia H. Wolff, one species in Notopterygium de Boiss. and one species in Trachydium Lindl. of Trib. Smyrnieae, and Turgenia latifolia ( Linn.) Hoffm. of Trib. Scandicineae,psbA-trnH fragment information of all species was analyzed, and MP, ML and BI phylogenetic trees were constructed taking T. latifolia as an outgroup. The results show that length of psbA-trnH fragment of both Chuanminshen violaceum and Changium smyrnioides is 258 bp and GC content of both is 23%, while length of psbA-trnH fragment of other species is 228-405 bp and their GC content is 26%-35%. And total length of psbA-trnH fragment after alignment is 553 bp ( including gaps) , in which, there are 237 variable sites and 178 information sites. The relative genetic distance between Chuanminshen violaceum and Changium smyrnioides is the smallest ( only 0 . 02 ) , while that among Chuanminshen violaceum and other species is 0. 10-1. 34, and generally, that among Chuanminshen violaceum and species in Trib. Smyrnieae is smaller, meaning that relationship of Chuanminshen violaceum with Changium smyrnioides and some species in Trib. Smyrnieae is nearer. In MP, ML and BI phylogenetic trees, both Chuanminshen violaceum and Changium smyrnioides are clustered together and are grouped as one clade with other species of Trib. Smyrnieae, and are away from another clade composed of species in Trib. Peucedaneae. Combined with characteristics of morphology and fruit anatomical structures, it is suggested that Chuanminshen Sheh et Shan should be separated from Trib. Peucedaneae and placed in Trib. Smyrnieae, and it is the sister taxa with Changium H. Wolff.