CAJ | 학술논문

为长柱金丝桃（Hypericumascyron L．）质量标准的研究提供参考，在色谱柱为Diamonsil C18（250 mm×4．6 mm，5"m）、流动相为甲醇乙腈0．6％磷酸水溶液、线性度（R2）高于0．9990、回收率为92．15％～108．37％、相对标准偏差为1．57％～1．91％的条件下，使用高效液相色谱法同时测定了延边地区长柱金丝桃中芦丁、金丝桃苷、槲皮素和山奈酚4种成分的含量．实验结果表明：长柱金丝桃中芦丁、金丝桃苷、槲皮素和山奈酚的含量随区域有较大差异；本文方法准确、灵敏、可靠．
위장주금사도（Hypericumascyron L．）질량표준적연구제공삼고，재색보주위Diamonsil C18（250 mm×4．6 mm，5"m）、류동상위갑순을정0．6％린산수용액、선성도（R2）고우0．9990、회수솔위92．15％～108．37％、상대표준편차위1．57％～1．91％적조건하，사용고효액상색보법동시측정료연변지구장주금사도중호정、금사도감、곡피소화산내분4충성분적함량．실험결과표명：장주금사도중호정、금사도감、곡피소화산내분적함량수구역유교대차이；본문방법준학、령민、가고．
To provide references for quality standard of Hypericumascyron L.,HPLC method was established for simultaneous determination of rutin,hyperoside,quercetin and kaempferol in Hypericum ascyron L.of Yanbian area.Chromatographic separation was employed on Diamonsil C18 column (250 mm×4.6 mm,5"m) with gradient elution of methanol-acetonitrile-0.6% phosphoric acid as mobile phase.Calibration curves of standard components show excellent linearity with R2>0.999 0 and the recovery tests are found to range from 92.15% to 108.37% with relative standard deviation values 1.57% 1.91%.The contents of flavonoids were diferent obviously in samples from diferent regions.This method is accurate,sensitive and reliable.