CAJ | 학술논문

目的：研究不同光周期下,MT1-R在无蹼壁虎室旁核(PVN)的节律性(昼夜节律和季节节律)表达变化。方法运用了免疫印迹、免疫组织化学和形态学计数法。结果(1)免疫印迹结果显示 MT1-R 蛋白分子量为37KD,MT1-R 阳性细胞主要分布在下丘脑室旁核,且有少量阳性纤维；(2)在无蹼壁虎的活动期(夏季),各光周期(DD、DL和 LL)下 MT1-R阳性细胞在PVN均呈明显的昼夜节律变化(P＜0.0001),DD、LL较DL均有时相变化；(3)在冬眠期(冬季),DL光周期下 MT1-R阳性细胞在PVN无明显的昼夜节律变化(P=0.005),DD和 LL光周期下 MT1-R阳性细胞在 PVN 呈明显的昼夜节律变化(P＜0.0001),DD、LL较DL均有时相变化；(4)各光周期(DD、DL和 LL)下 MT1-R阳性细胞在PVN均呈明显的季节性节律变化(P＜0.0001),活动期(夏季)的 MT1-R阳性细胞数较冬眠期(冬季)明显多(P＜0.0001),DD和 LL 在活动期、冬眠期的节律图形相似。结论 MT 1-R在无蹼壁虎室旁核的表达有明显的节律性(昼夜节律和季节节律),不同光周期对 MT 1-R的节律性表达有影响,但其图形基本相似。提示光周期可能通过启动“内源性节律钟”影响 MT 1-R在无蹼壁虎室旁核的节律性表达,其可能调控 MT 1-R蛋白的表达量。
목적：연구불동광주기하,MT1-R재무복벽호실방핵(PVN)적절률성(주야절률화계절절률)표체변화。방법운용료면역인적、면역조직화학화형태학계수법。결과(1)면역인적결과현시 MT1-R 단백분자량위37KD,MT1-R 양성세포주요분포재하구뇌실방핵,차유소량양성섬유；(2)재무복벽호적활동기(하계),각광주기(DD、DL화 LL)하 MT1-R양성세포재PVN균정명현적주야절률변화(P＜0.0001),DD、LL교DL균유시상변화；(3)재동면기(동계),DL광주기하 MT1-R양성세포재PVN무명현적주야절률변화(P=0.005),DD화 LL광주기하 MT1-R양성세포재 PVN 정명현적주야절률변화(P＜0.0001),DD、LL교DL균유시상변화；(4)각광주기(DD、DL화 LL)하 MT1-R양성세포재PVN균정명현적계절성절률변화(P＜0.0001),활동기(하계)적 MT1-R양성세포수교동면기(동계)명현다(P＜0.0001),DD화 LL 재활동기、동면기적절률도형상사。결론 MT 1-R재무복벽호실방핵적표체유명현적절률성(주야절률화계절절률),불동광주기대 MT 1-R적절률성표체유영향,단기도형기본상사。제시광주기가능통과계동“내원성절률종”영향 MT 1-R재무복벽호실방핵적절률성표체,기가능조공 MT 1-R단백적표체량。
Obj ective In the different photoperiod,we study rhythmicity(circadian rhythm and sea-sonal rhythm)expression change of MT1-R in the paraventricular nucleus of Gecko Swinhonis.Methods We use the Western blot,immunohisto chemistry and morphology of counting method.Results (1)West-ern blot showed that MT1-R protein with a molecular weight of 37KD,MT1-R positive cells were mainly distributed in the hypothalamic paraventricular nucleus,and a small amount of immunoreactive fibers;(2) In activity period (summer )of Gecko Swinhonis,the light cycle (DD,DL and LL )of MT1-R positive cells in PVN showed obvious changes in circadian rhythms (P<0.0001 ),DD and LL than DL are some-times phase change;(3)During the hibernation season (winter ),DL light cycle of MT1-R positive cells in PVN had no obvious changes in circadian rhythms (p =0.005 ),DD and LL light cycle of MT1-R posi-tive cells in PVN was obviously changes in circadian rhythms (P<0.0001 ),DD and LL than DL are sometimes phase change;(4)The light cycle (DD,DL and LL )of MT1-R positive cells in PVN showed clear seasonal rhythm (P<0.0001 ).The number of MT1-R positive cells is active period (summer )more than a period of hibernation(winter )(P<0.0001 ),DD and LL in active stage,hibernation rhythms graph similarity.Conclusion In different photoperiod,MT1-R in the paraventricular nucleus of the expres-sion of a clear rhythm (circadian rhythm and seasonal rhythm ),different light cycle on MT1-R rhythmic-ity expression have effect,but its basic graphs similar.It is suggested that light cycle may through the start of"endogenous circadian clock"effect of MT1-R Gecko Swinhonis in the paraventricular nucleus of the rhythmicity expression,which can only control the expression of MT1-R protein content.