CAJ | 학술논문

目的：检测肺鳞状细胞癌中CD44蛋白和 maspin蛋白的表达及相互之间的关系,并研究它们与肺鳞状细胞癌患者临床病理因素之间的关系。方法采用免疫组织化学Elivision法检测116例肺鳞状细胞癌和30例正常肺组织中CD44、maspin和CD34蛋白表达情况。结果在肺鳞状细胞癌和正常肺组织中 CD44和 maspin蛋白的阳性表达率分别为60.3%、39.7%和10.0%、100%,两组之间相比,差异有统计学意义；CD44和 maspin 蛋白的阳性表达率和微血管密度(microvessel density MVD)与肺鳞状细胞癌患者肿瘤组织的分化级别、淋巴结转移与否以及临床分期等相关(P＜0.05)；且 CD44蛋白阳性表达率与 MVD计数呈正相关关系,maspin蛋白阳性表达与CD44蛋白阳性表达和 MVD计数均呈负相关关系(P＜0.05)。Kaplan-Meier生存分析显示,CD44蛋白阳性表达组患者的生存时间明显高低于其阴性表达组患者(P＜0.001)；maspin蛋白阳性表达组患者的生存时间明显高于其阴性表达组患者(P＜0.001)；MVD≥21组患者生存时间明显低于 MVD＜21组的患者(P＜0.001)。多因素分析表明CD44和 maspin蛋白的表达是肺鳞状细胞癌患者的独立预后因素。结论 CD44和 maspin的异常表达参与了肺鳞状细胞癌的发生、发展、浸润和转移,联合检测 CD44和 maspin蛋白的表达对肺鳞状细胞癌的进展和预后判断有重要意义。
목적：검측폐린상세포암중CD44단백화 maspin단백적표체급상호지간적관계,병연구타문여폐린상세포암환자림상병리인소지간적관계。방법채용면역조직화학Elivision법검측116례폐린상세포암화30례정상폐조직중CD44、maspin화CD34단백표체정황。결과재폐린상세포암화정상폐조직중 CD44화 maspin단백적양성표체솔분별위60.3%、39.7%화10.0%、100%,량조지간상비,차이유통계학의의；CD44화 maspin 단백적양성표체솔화미혈관밀도(microvessel density MVD)여폐린상세포암환자종류조직적분화급별、림파결전이여부이급림상분기등상관(P＜0.05)；차 CD44단백양성표체솔여 MVD계수정정상관관계,maspin단백양성표체여CD44단백양성표체화 MVD계수균정부상관관계(P＜0.05)。Kaplan-Meier생존분석현시,CD44단백양성표체조환자적생존시간명현고저우기음성표체조환자(P＜0.001)；maspin단백양성표체조환자적생존시간명현고우기음성표체조환자(P＜0.001)；MVD≥21조환자생존시간명현저우 MVD＜21조적환자(P＜0.001)。다인소분석표명CD44화 maspin단백적표체시폐린상세포암환자적독립예후인소。결론 CD44화 maspin적이상표체삼여료폐린상세포암적발생、발전、침윤화전이,연합검측 CD44화 maspin단백적표체대폐린상세포암적진전화예후판단유중요의의。
Obj ective To explore the expression of CD44 and maspin proteins in the lung squamous cell carcinoma (LSCC)and their relationship with microvessel density (MVD).Methods The expression of CD44, maspin,and CD34 proteins was examined by immunohistochemistry in 116 specimens of LSCC and 30 normal lung tissues.Results The positive expression of CD44 and maspin in normal lung tissues was 10.0% and 100%, but 60.3% and 39.7%,respectively in LSCC tissues.There was significant differences between the two groups (P<0.05).The expression of CD44 and maspin proteins was significantly related with grades of tumors,lymph node metastasis,and TNM stages,as was the score of MVD (P<0.05).There was a pos-itive relationship between the expression of CD44 protein and the score of MVD (P<0.001);and the ex-pression of maspin protein was negatively associated with the expression of CD44 protein and the score of MVD (P<0.001).Kaplan-Meier survival analysis:showed that the overall mean survival time of patients with positive CD44 protein expression was shorter than that of those with negative expression (P<0.001);the survival time patients with positive maspin expression was significantly longer than that of those with negative expression (P<0001);the survival time of patients with ≥21 was significantly shor-ter than that of those with MVD<21 group (P<0.001).Positive expressions of CD44 and maspin were independent prognostic factors of LSCC (P<0.05).Conclusion Abnormal expression of CD44 and maspin plays an important role in the initiation,progression,and metastasis of LSCC.The combined detection of CD44 and maspin has an important role in predicting the progression and prognosis of LSCC.