CAJ | 학술논문

目的：研究PAX-5、CD117、PDGFRA在胃肠道神经内分泌肿瘤中的表达,并分析与肿瘤生物学特点及患者生存时间的关系。方法应用组织芯片及免疫组化法,对96例胃肠道神经内分泌肿瘤的免疫表型和预后进行分析。结果PAX-5、CD117、PDGFRA蛋白的阳性率分别为31.3%、19.8%、53.1%。PAX-5和 PDGFRA 表达与胃肠道神经内分泌肿瘤类型、肿瘤大小、TNM分期及淋巴结转移有显著相关性,CD117表达与TNM分期及淋巴结转移有显著相关性。TNM分期、淋巴结转移、肿瘤大小、CD117、P53、Ki-67表达与生存时间呈负相关,但只有 TNM分期、CD117表达和 Ki-67高表达为影响预后的独立因素。结论 PAX-5、CD117、PDGFRA的表达可能与胃肠道神经内分泌肿瘤的发生发展有关,CD117表达和 Ki-67高表达是独立预后因子,PAX-5可以作为潜在的分子治疗靶点。
목적：연구PAX-5、CD117、PDGFRA재위장도신경내분비종류중적표체,병분석여종류생물학특점급환자생존시간적관계。방법응용조직심편급면역조화법,대96례위장도신경내분비종류적면역표형화예후진행분석。결과PAX-5、CD117、PDGFRA단백적양성솔분별위31.3%、19.8%、53.1%。PAX-5화 PDGFRA 표체여위장도신경내분비종류류형、종류대소、TNM분기급림파결전이유현저상관성,CD117표체여TNM분기급림파결전이유현저상관성。TNM분기、림파결전이、종류대소、CD117、P53、Ki-67표체여생존시간정부상관,단지유 TNM분기、CD117표체화 Ki-67고표체위영향예후적독립인소。결론 PAX-5、CD117、PDGFRA적표체가능여위장도신경내분비종류적발생발전유관,CD117표체화 Ki-67고표체시독립예후인자,PAX-5가이작위잠재적분자치료파점。
Obj ective To investigate the expression of PAX-5 ,CD1 1 7 and PDGFRA in gastrointesti-nal neuroendocrine neoplasm (GINEN)and their relationship to tumor biological characteristics and sur-vival time of patients.Methods Ninety-six cases in tissue microarray were studied by immunohistochemis-try.The relationship between the expression of PAX-5,CD117,PDGFRA and the clinicopathological fac-tors was analyzed by Chi-square test,multivariate analysis and Kaplan-Meier method.Results The posi-tive expression rate of PAX-5 ,CD1 1 7 and PDGFRA in GINEN was 3 1 .3%,1 9 .8% and 5 3 .1%.Both PAX-5 and PDGFRA related to pathological type,tumor size,TNM stage,and lymph node metastasis (P<0.05);while CD117 related to TNM stage and lymph node metastasis (P<0.05).Besides the clinico-pathological factors including TNM stage,lymph node metastasis and tumor size,the expression of CD117,P53 and Ki-67 also showed negative relationships to prognosis in Kaplan-Meier survival analysis (P<0.05);but Cox survival analysis demonstrated that only TNM stage,CD117 expression and high Ki-67 index were associated with survival time of patients (P<0.05).Conclusion The expression of PAX-5, CD1 1 7 and PDGFRA plays important roles in the pathogenesis of GINEN.Both CD1 1 7 expression and high Ki-67 index are independent prognostic factors.PAX-5 could be a potential target for therapy.