中国组织化学与细胞化学杂志
中國組織化學與細胞化學雜誌
중국조직화학여세포화학잡지
CHINESE JOURNAL OF HISTOCHEMISY AND CYTOCHEMISY
2014年
3期
242-247
,共6页
胃肿瘤%MDM2%幽门螺杆菌%L型
胃腫瘤%MDM2%幽門螺桿菌%L型
위종류%MDM2%유문라간균%L형
Gastric neoplasm%MDM2%Helicobacter Pylori%L-form
目的：研究 MDM2在胃癌组织中的表达意义及其与幽门螺杆菌 L型(Helicobacter pylori-L,Hp-L)感染在胃癌发生中的关系。方法(1)应用革兰染色法和免疫组化学法检测100例胃癌及对应的40例癌旁正常胃黏膜组织(对照组)中Hp-L型感染和 MDM2蛋白的表达情况；(2)应用逆转录多聚酶链反应(RT-PCR)方法检测 MDM2的 mRNA在30例新鲜胃癌组织及其对应的癌旁正常胃粘膜组织(对照组)中的表达情况。结果100例胃癌组织及对应的40例癌旁正常胃粘膜组织中 MDM2阳性表达率为71.0%(71/100)和42.5%(17/40),可见 MDM2在胃癌组织中高表达和在非胃癌组中低表达具有统计学意义(P＜0.05),且 MDM2表达水平升高与肿瘤大小、浸润深度、淋巴结转移和TNM分期有关(P＜0.05),与性别、年龄无关(P＞0.05)；RT-PCR显示,肿瘤组织 MDM2的表达明显高于远端正常胃黏膜对照组织的表达量且差异明显(P＜0.01)。革兰染色和免疫组化两种方法检测 Hp-L型结果具有一致性(P＞0.05),胃癌组织中 Hp-L型感染阳性组的 MDM2表达阳性率高于 Hp-L型阴性组(P＜0.05),且 Hp-L型阳性率和MDM2蛋白表达呈正相关(r=0.447,P＜0.05)。结论 MDM2蛋白在胃癌中呈高表达,且与胃癌的浸润、转移有关,其机制可能与 Hp-L型感染有关。
目的：研究 MDM2在胃癌組織中的錶達意義及其與幽門螺桿菌 L型(Helicobacter pylori-L,Hp-L)感染在胃癌髮生中的關繫。方法(1)應用革蘭染色法和免疫組化學法檢測100例胃癌及對應的40例癌徬正常胃黏膜組織(對照組)中Hp-L型感染和 MDM2蛋白的錶達情況；(2)應用逆轉錄多聚酶鏈反應(RT-PCR)方法檢測 MDM2的 mRNA在30例新鮮胃癌組織及其對應的癌徬正常胃粘膜組織(對照組)中的錶達情況。結果100例胃癌組織及對應的40例癌徬正常胃粘膜組織中 MDM2暘性錶達率為71.0%(71/100)和42.5%(17/40),可見 MDM2在胃癌組織中高錶達和在非胃癌組中低錶達具有統計學意義(P＜0.05),且 MDM2錶達水平升高與腫瘤大小、浸潤深度、淋巴結轉移和TNM分期有關(P＜0.05),與性彆、年齡無關(P＞0.05)；RT-PCR顯示,腫瘤組織 MDM2的錶達明顯高于遠耑正常胃黏膜對照組織的錶達量且差異明顯(P＜0.01)。革蘭染色和免疫組化兩種方法檢測 Hp-L型結果具有一緻性(P＞0.05),胃癌組織中 Hp-L型感染暘性組的 MDM2錶達暘性率高于 Hp-L型陰性組(P＜0.05),且 Hp-L型暘性率和MDM2蛋白錶達呈正相關(r=0.447,P＜0.05)。結論 MDM2蛋白在胃癌中呈高錶達,且與胃癌的浸潤、轉移有關,其機製可能與 Hp-L型感染有關。
목적：연구 MDM2재위암조직중적표체의의급기여유문라간균 L형(Helicobacter pylori-L,Hp-L)감염재위암발생중적관계。방법(1)응용혁란염색법화면역조화학법검측100례위암급대응적40례암방정상위점막조직(대조조)중Hp-L형감염화 MDM2단백적표체정황；(2)응용역전록다취매련반응(RT-PCR)방법검측 MDM2적 mRNA재30례신선위암조직급기대응적암방정상위점막조직(대조조)중적표체정황。결과100례위암조직급대응적40례암방정상위점막조직중 MDM2양성표체솔위71.0%(71/100)화42.5%(17/40),가견 MDM2재위암조직중고표체화재비위암조중저표체구유통계학의의(P＜0.05),차 MDM2표체수평승고여종류대소、침윤심도、림파결전이화TNM분기유관(P＜0.05),여성별、년령무관(P＞0.05)；RT-PCR현시,종류조직 MDM2적표체명현고우원단정상위점막대조조직적표체량차차이명현(P＜0.01)。혁란염색화면역조화량충방법검측 Hp-L형결과구유일치성(P＞0.05),위암조직중 Hp-L형감염양성조적 MDM2표체양성솔고우 Hp-L형음성조(P＜0.05),차 Hp-L형양성솔화MDM2단백표체정정상관(r=0.447,P＜0.05)。결론 MDM2단백재위암중정고표체,차여위암적침윤、전이유관,기궤제가능여 Hp-L형감염유관。
Obj ective To investigate the expression of the MDM2 protein in gastric carcinoma and its relationship with the Helicobacter pylori L-form (Hp-L)infection.Methods (1)Immunohistochemical and Gram-staining were used to detect the Hp-L infection and the expression of MDM2 protein in 100 samples of gastric cancer tissue and 40 samples of the corresponding adjacent tissues (control group).(2)The ex-pression of MDM2 mRNA in 30 cases of fresh gastric cancer tissue and the correspording normal gastric mucosa (control group)were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).Results The rate of positive expressions of MDM2 gene in gastric cancers and adj acent tissue were 71.0%(71/100)and 42.5%(17/40),the positive rate of MDM2 protein expression in gastric carcino-ma was significantly higher than in the control group (P<0.05),MDM2 protein expression increased with tumor size,depth of invasion,lymph node metastasis and TNM stage (P <0.05),not correlated with pa-tients'sex and age (P>0.05 ).RT-PCR showed significant differences MDM2 expression in between tumor tissue and distal normal control tissues(P<0.01).The results by Gramstaining and immunohis to-chemistry for Hp-L type were consistent (P>0.05).In gastric cancer,group ,the expression of MDM2 was significantly higher in the Hp-L positive group than that in negative group (P<0.05).There was a significant positive relationship of Hp-L with MDM2 (r=0.447,P<<0.05).Conclusion The high ex-pression of MDM2 protein and mRNA in gastric cancer was increased and correlated with tumor invasion and metastasis,and the mechanism may be related to Helicobacter pylori L-form infection.
