西南林业大学学报
西南林業大學學報
서남임업대학학보
JOURNAL OF SOUTHWEST FORESTRY COLLEGE
2014年
3期
7-11
,共5页
吴岐奎%邢世岩%范圣明%王萱%孙立民%刘晓静
吳岐奎%邢世巖%範聖明%王萱%孫立民%劉曉靜
오기규%형세암%범골명%왕훤%손립민%류효정
垂乳银杏%种质资源%亲缘关系%遗传多样性%AFLP
垂乳銀杏%種質資源%親緣關繫%遺傳多樣性%AFLP
수유은행%충질자원%친연관계%유전다양성%AFLP
Ginkgo biloba chichi%germplasm resources%relationship%genetic diversity%AFLP
采用AFLP分子标记技术对14份垂乳银杏种质资源进行遗传多样性和亲缘关系分析。结果表明,8对引物在14份垂乳银杏种质扩增后检测出935条谱带,有920条多态带,多态带比例为98.40%,表明所试垂乳银杏材料具有丰富的多样性;遗传相似系数为0.4404~0.7299,平均值为0.4752,说明各种质之间有一定的遗传分化;聚类分析和主坐标分析将垂乳银杏分成5类,来源相同的种质并没有严格的聚在一起,表明垂乳银杏的变异与地理来源无严格的相关性。
採用AFLP分子標記技術對14份垂乳銀杏種質資源進行遺傳多樣性和親緣關繫分析。結果錶明,8對引物在14份垂乳銀杏種質擴增後檢測齣935條譜帶,有920條多態帶,多態帶比例為98.40%,錶明所試垂乳銀杏材料具有豐富的多樣性;遺傳相似繫數為0.4404~0.7299,平均值為0.4752,說明各種質之間有一定的遺傳分化;聚類分析和主坐標分析將垂乳銀杏分成5類,來源相同的種質併沒有嚴格的聚在一起,錶明垂乳銀杏的變異與地理來源無嚴格的相關性。
채용AFLP분자표기기술대14빈수유은행충질자원진행유전다양성화친연관계분석。결과표명,8대인물재14빈수유은행충질확증후검측출935조보대,유920조다태대,다태대비례위98.40%,표명소시수유은행재료구유봉부적다양성;유전상사계수위0.4404~0.7299,평균치위0.4752,설명각충질지간유일정적유전분화;취류분석화주좌표분석장수유은행분성5류,래원상동적충질병몰유엄격적취재일기,표명수유은행적변이여지리래원무엄격적상관성。
The genetic diversity and relationship of 14 Ginkgo biloba chichi clones were tested by AFLP.8 primer combinations selected from 64 primer combinations produce a total of 935 bands,of which 920 bands were polymorphic,and accounted for 98.40%.It suggested that Ginkgo biloba chichi has a rich genetic diversity.The molecular genetic similarity coefficients ranged from 0.440 4 to 0.729 9,and that the average being 0.475 2,indi-cating that genetic variation occurs among them.All of Ginkgo biloba chichi were clustered into 5 groups according to UPGMA cluster and principal coordinate analysis,but the clones from the same origin didn′t distribute in the same group totally.The result indicated that there′s no strict correlation between geographical and genetic diversity.