CAJ | 학술논문

采用化学聚合方法制备透明质酸(HA)掺杂的聚3,4-乙撑二氧噻吩(PEDOT)导电材料，通过正交实验优化反应条件(掺杂剂、氧化剂、反应时间)提高其电导率，将其与聚左乳酸(PLLA)结合，制得一种导电性良好且可生物降解的PEDOT/PLLA复合材料，考察了PC12细胞在PEDOT/PLLA膜上的粘附生长等生物相容性指标。四探针电导率仪检测表明，当HA为0.05 g，过硫酸铵(APS)为0.015 mol，反应时间为24 h，制备的PEDOT电导率可达0.36 S?cm-1；通过傅里叶变换红外光谱(FT-IR)、能谱(EDS)分析掺杂所得PEDOT纳米材料成分，以及扫描电镜(SEM)观察PEDOT颗粒和PEDOT/PLLA复合薄膜的表面形态，表明HA已成功掺杂到PEDOT，且制得的PEDOT/PLLA复合薄膜中PEDOT分散性较好；荧光显微镜、金相显微镜观察及CCK-8分析表明，PEDOT颗粒有利于PC12细胞的粘附及突触伸长，且在包被层粘连蛋白(LN)的PEDOT/PLLA膜(质量浓度为30%(wt))培养72 h后，细胞存活率增加至对照的(116.6±3.2)%。以上结果初步表明,PEDOT/PLLA膜具有较好的生物相容性，且利于PC12细胞突触的伸长，这为进一步神经组织工程导电生物支架的研究制备提供了实验支持。
채용화학취합방법제비투명질산(HA)참잡적취3,4-을탱이양새분(PEDOT)도전재료，통과정교실험우화반응조건(참잡제、양화제、반응시간)제고기전도솔，장기여취좌유산(PLLA)결합，제득일충도전성량호차가생물강해적PEDOT/PLLA복합재료，고찰료PC12세포재PEDOT/PLLA막상적점부생장등생물상용성지표。사탐침전도솔의검측표명，당HA위0.05 g，과류산안(APS)위0.015 mol，반응시간위24 h，제비적PEDOT전도솔가체0.36 S?cm-1；통과부리협변환홍외광보(FT-IR)、능보(EDS)분석참잡소득PEDOT납미재료성분，이급소묘전경(SEM)관찰PEDOT과립화PEDOT/PLLA복합박막적표면형태，표명HA이성공참잡도PEDOT，차제득적PEDOT/PLLA복합박막중PEDOT분산성교호；형광현미경、금상현미경관찰급CCK-8분석표명，PEDOT과립유리우PC12세포적점부급돌촉신장，차재포피층점련단백(LN)적PEDOT/PLLA막(질량농도위30%(wt))배양72 h후，세포존활솔증가지대조적(116.6±3.2)%。이상결과초보표명,PEDOT/PLLA막구유교호적생물상용성，차리우PC12세포돌촉적신장，저위진일보신경조직공정도전생물지가적연구제비제공료실험지지。
Conductive HA (Hyaluronic Acid)-doped PEDOT (Poly 3,4-ethylenedioxythiophene) particles were synthesized through chemical polymerization and an orthogonal experiment was designed to improve the conductivity of these PEDOT particles. The results show that when the experiment is conducted under conditions of HA 0.05 g, ammonium persulfate (APS ) 0.015 mol and reaction time of 24 hours, the optimized conductivity measured by four-point probe can reach to 0.36 S·cm-1. A conductive biodegradable PEDOT/PLLA composite membrane was prepared by incorporating HA-doped PEDOT particles to biodegradable PLLA (Poly L, lactic acid). FT-IR and EDX results confirm the presence of HA on the PEDOT particles, and the surface morphology of PEDOT particles and the PEDOT/PLLA composite membrane were observed with SEM. PC12 cells were cultured on the 30%PEDOT/PLLA membrane modified by LN (laminin protein) and the cells demonstrate normal morphology, high adhesion and longer cell synaptic attachment on the membrane. Compared with the control group, PEDOT/PLLA membrane can effectively increase cell viability by (116.6±3.2)%after 72 hour. The results show that the conductive biodegradable PEDOT/PLLA membrane is useful in nervous tissue engineering for preparing conductive biodegradable scaffolds.