广西大学学报(自然科学版)
廣西大學學報(自然科學版)
엄서대학학보(자연과학판)
JOURNAL OF GUANGXI UNIVERSITY (NATURAL SCIENCE EDITION)
2014年
3期
461-466
,共6页
乔宽%韦万兴%莫利书%王奎武%赵树凯
喬寬%韋萬興%莫利書%王奎武%趙樹凱
교관%위만흥%막리서%왕규무%조수개
雀梅藤%果实%花青素%分离%稳定性
雀梅籐%果實%花青素%分離%穩定性
작매등%과실%화청소%분리%은정성
Sageretia theezans Brongn%fruit%anthocyanin%separation%stability
为开发利用雀梅藤果实中的花青素资源,采用含0.001 mol/L盐酸的水溶液对可食的雀梅藤果实进行花青素粗提液提取,利用阳离子交换树脂从花青素粗提液中分离得到雀梅浆果花青素( Sageretia theezans Brongn fruits anthocyanins, STFA),然后采用LC-MS对雀梅浆果花青素的组成成分进行分析,最后对雀梅浆果花青素的稳定性进行了研究。结果表明,雀梅浆果花青素成分包括矢车菊色素-3-槐糖-5-葡萄糖苷、矮牵牛素-3-(6'-丙二酰)葡萄糖苷、锦葵素-3-葡萄糖苷和芍药素-3-(6'-丙二酰)葡萄糖苷,其在总花青素中的百分比分别为0.38%,22.57%,44.32%和30.86%;紫外光、红外光和室内自然光对花青素稳定性的影响较小,温度对花青素稳定性的影响较大,当pH≤6时有利于花青素的保存。研究结果可为雀梅浆果花青素的工业化生产提供参考依据。
為開髮利用雀梅籐果實中的花青素資源,採用含0.001 mol/L鹽痠的水溶液對可食的雀梅籐果實進行花青素粗提液提取,利用暘離子交換樹脂從花青素粗提液中分離得到雀梅漿果花青素( Sageretia theezans Brongn fruits anthocyanins, STFA),然後採用LC-MS對雀梅漿果花青素的組成成分進行分析,最後對雀梅漿果花青素的穩定性進行瞭研究。結果錶明,雀梅漿果花青素成分包括矢車菊色素-3-槐糖-5-葡萄糖苷、矮牽牛素-3-(6'-丙二酰)葡萄糖苷、錦葵素-3-葡萄糖苷和芍藥素-3-(6'-丙二酰)葡萄糖苷,其在總花青素中的百分比分彆為0.38%,22.57%,44.32%和30.86%;紫外光、紅外光和室內自然光對花青素穩定性的影響較小,溫度對花青素穩定性的影響較大,噹pH≤6時有利于花青素的保存。研究結果可為雀梅漿果花青素的工業化生產提供參攷依據。
위개발이용작매등과실중적화청소자원,채용함0.001 mol/L염산적수용액대가식적작매등과실진행화청소조제액제취,이용양리자교환수지종화청소조제액중분리득도작매장과화청소( Sageretia theezans Brongn fruits anthocyanins, STFA),연후채용LC-MS대작매장과화청소적조성성분진행분석,최후대작매장과화청소적은정성진행료연구。결과표명,작매장과화청소성분포괄시차국색소-3-괴당-5-포도당감、왜견우소-3-(6'-병이선)포도당감、금규소-3-포도당감화작약소-3-(6'-병이선)포도당감,기재총화청소중적백분비분별위0.38%,22.57%,44.32%화30.86%;자외광、홍외광화실내자연광대화청소은정성적영향교소,온도대화청소은정성적영향교대,당pH≤6시유리우화청소적보존。연구결과가위작매장과화청소적공업화생산제공삼고의거。
In order to exploit and utilize the anthocyanin components from fruit of Sageretia theezans Brongn fruits, the edible fruit of Sageretia theezans Brongn was extracted by aqueous solution of HCl (0.001 mol/L) to give crude anthocyanin. The crude anthocyanin were separated to obtain Sagere-tia theezans Brongn fruits anthocyanins ( STFA) by cation ion-exchange resin. Results showed that STFA was Cy-3-Sp-5-Glu, Pt-3-(6’-Mal) Glu, Mv-3-Glu, Pn-3-(6’-Mal) Glu and their relative con-tent obtained by LC-MS analysis were 0.38%, 22.57%, 44.32%, 30.86% respectively. Antho-cyanins were stable under ultraviolet ray, infrared ray and indoor natural light in a short interval, but not stable in high temperature and high pH value. These findings provide an important basis for STFA industrial process.