CAJ | 학술논문

为研究海洋真菌萨氏曲霉( Aspergillus sydowii) MNP12010103的次级代谢产物及其抗肿瘤活性,采用活性导向方法以及大孔树脂、硅胶、Sephedax LH-20、半制备高效液相色谱等方法对其进行分离纯化,并经波谱分析对其进行结构鉴定。结果分离得到7个化合物,分别鉴定为：环(亮氨酸-脯氨酸)(化合物1),1,8二羟基蒽醌(化合物2),5吲哚醛(化合物3),大黄素6甲醚(化合物4),5α胆甾烷3酮(化合物5),尿嘧啶(化合物6),胸苷(化合物7)。其中,5α胆甾烷3酮(化合物5)为首次从微生物中分离得到；1,8二羟基蒽醌(化合物2)为首次从海洋真菌中分离得到；化合物1、化合物3和化合物4为首次从该菌株中分离得到。化合物2抑制PANC-1细胞的IC50值为45.47μg/mL；化合物4抑制PC-3细胞的IC50值为53.97μg/mL；化合物2和化合物4浓度为100μg/mL时,对DPPH自由基的清除率均超过50%。
위연구해양진균살씨곡매( Aspergillus sydowii) MNP12010103적차급대사산물급기항종류활성,채용활성도향방법이급대공수지、규효、Sephedax LH-20、반제비고효액상색보등방법대기진행분리순화,병경파보분석대기진행결구감정。결과분리득도7개화합물,분별감정위：배(량안산-포안산)(화합물1),1,8이간기은곤(화합물2),5신타철(화합물3),대황소6갑미(화합물4),5α담치완3동(화합물5),뇨밀정(화합물6),흉감(화합물7)。기중,5α담치완3동(화합물5)위수차종미생물중분리득도；1,8이간기은곤(화합물2)위수차종해양진균중분리득도；화합물1、화합물3화화합물4위수차종해균주중분리득도。화합물2억제PANC-1세포적IC50치위45.47μg/mL；화합물4억제PC-3세포적IC50치위53.97μg/mL；화합물2화화합물4농도위100μg/mL시,대DPPH자유기적청제솔균초과50%。
To study the secondary metabolites and their antitumor activity of the marine fungus As-pergillus sydowii MNP12010103, column chromatography on macroporous resin, silica gel, Sepha-dex-LH20 , PHPLC were used to isolate the compounds from the marine fungus MNP12010103 using bioactivity guide method. The structures of these compounds were identified by spectroscopic analy-sis. Seven compounds obtained were identified as cyclo-(Leu-Pro) (compound 1), 1, 8-dihydroxy anthraquinone ( compound 2 ) , indole-5-carboxaldehyde ( compound 3 ) , physcion ( compound 4 ) , 5α-cholestane-3-one ( compound 5 ) , uracil ( compound 6 ) , and thymidine ( compound 7 ) . Com-pound 5 was isolated from microorganism for the first time, compound 2 from marine fungus for the first time, and compound 1,3,4 from the fungi of Aspergillus sydowii for the first time. Compounds 2 exhibits inhibitory activities on PANC-1 with IC50 of 45.47 μg/mL and compounds 4 shows cytotox-icity against PC-3 with IC50 of 53.97μg/mL. The DPPH radical scavenging rate of both compound 2 and compound 4 are more than 50% at a concentration of 100 μg/mL.