CAJ | 학술논문

比较了8种大孔吸附树脂D101、AB-8、NKA-9、D4020、S-8、200702、H103、NKA-Ⅱ对菊米总黄酮的吸附性能，以大孔吸附树脂对菊米总黄酮的吸附率、洗脱率为评价指标，筛选出合适的大孔吸附树脂分离纯化菊米总黄酮,并以静态实验、动态试验考察大孔树脂对菊米总黄酮的分离纯化效果及影响因素，优化吸附和解吸条件。结果表明：200702中极性树脂分离纯化菊米总黄酮效果较好，其最佳吸附工艺为：上样液pH 5~6，质量浓度0.35 mg?mL-1，上样液流速3.0 mL?min-1，最佳洗脱工艺为：70％乙醇溶液30 mL，洗脱速率2.5 mL?min-1，通过本工艺菊米总黄酮纯度达83.5％。
비교료8충대공흡부수지D101、AB-8、NKA-9、D4020、S-8、200702、H103、NKA-Ⅱ대국미총황동적흡부성능，이대공흡부수지대국미총황동적흡부솔、세탈솔위평개지표，사선출합괄적대공흡부수지분리순화국미총황동,병이정태실험、동태시험고찰대공수지대국미총황동적분리순화효과급영향인소，우화흡부화해흡조건。결과표명：200702중겁성수지분리순화국미총황동효과교호，기최가흡부공예위：상양액pH 5~6，질량농도0.35 mg?mL-1，상양액류속3.0 mL?min-1，최가세탈공예위：70％을순용액30 mL，세탈속솔2.5 mL?min-1，통과본공예국미총황동순도체83.5％。
Based on static adsorption and desorption rates, adsorption and desorption characteristics of macroporous resins D101, AB-8, NKA-9, D4020, S-8, 200702, H103, and NKA-Ⅱ used for the separation of flavonoids from Jumi were studied. The most suitable macroporous resin was selected, and then static and dynamic adsorption experiments were employed to optimize the separation and purification conditions.The results show that the 200702 middle polar macroporous resin offers the best adsorption and desorption behaviors for total flavonoids from Jumi. The optimum adsorption conditions are: sample pH 5-6, sample content 0.35 mg?mL-1 and sample loading rate 3.0 mL?min-1. The optimum desorption conditions are:30 mL of 70% ethanol solution as the eluting solvent with an eluting rate of 2.5 mL?min-1. The purity of the total flavonoids from Jumi reaches to 83.5% under these conditions.