CAJ | 학술논문

目的：探讨人参皂苷Rg1（G-Rg1）对环孢素A（CsA）慢性肾毒性大鼠肾脏肾小管上皮-间充质细胞转分化（EMT）的抑制作用。方法雄性SD大鼠24只，随机分为3组：正常组、模型组、G-Rg1干预组，采用低盐饮食加CsA灌胃的方法建立CsA慢性肾纤维化模型，CsA剂量为25 mg·kg-1·d-1。观察G-Rg1对大鼠尿量、肾功能、肾脏病理及肾脏转化生长因子（TGF）-β1、E-钙黏素、α-平滑肌肌动蛋白（SMA）表达的影响。结果 G-Rg1能抑制CsA慢性肾毒性大鼠肾脏纤维化，下调TGF-β1、α-SMA在肾组织的表达，同时上调肾组织E-钙黏素的表达。结论 G-Rg1对CsA慢性肾毒性大鼠具有保护作用，其机制可能是抑制了肾组织的EMT途径。
목적：탐토인삼조감Rg1（G-Rg1）대배포소A（CsA）만성신독성대서신장신소관상피-간충질세포전분화（EMT）적억제작용。방법웅성SD대서24지，수궤분위3조：정상조、모형조、G-Rg1간예조，채용저염음식가CsA관위적방법건립CsA만성신섬유화모형，CsA제량위25 mg·kg-1·d-1。관찰G-Rg1대대서뇨량、신공능、신장병리급신장전화생장인자（TGF）-β1、E-개점소、α-평활기기동단백（SMA）표체적영향。결과 G-Rg1능억제CsA만성신독성대서신장섬유화，하조TGF-β1、α-SMA재신조직적표체，동시상조신조직E-개점소적표체。결론 G-Rg1대CsA만성신독성대서구유보호작용，기궤제가능시억제료신조직적EMT도경。
Objective To investigate the effects of ginsenoside Rg1 (G-Rg1) on renal epithelial-mesenchymal transformation (EMT) in rats with chronic cyclosporine A (CsA) nephrotoxicity. Methods Twenty-four male SD rats were randomly assigned to normal control group, model control group and G-Rg1 treatment group. Low-salt diet and CsA were administered by gastric injection at the dose of 25 mg·kg-1·d-1 for establishing the models of chronic CsA nephrotoxicity. The effects of G-Rg1 on the volume of urine, renal function, renal pathophysioiogy and renal TGF-β1, E-cadherin and α-SMA expressions were determined. Results G-Rg1 down-regulated TGF-β1 and α-SMA and up-regulated E-cadherin expressions in renal tissues in rats with chronic CsA nephmtoxicity. Conclusion G-Rg1 harbors protective effects to the kidneys in rats possibly via inhibition of the EMT process.