CAJ | 학술논문

目的：优化五味子木脂素超声辅助提取工艺，获取工艺参数。方法利用响应面法(RSM)对五味子木脂素超声辅助乙醇提取工艺进行优化。在单因素实验基础上，选择乙醇浓度、超声提取时间和液固比为自变量，木脂素提取率为响应值，根据中心组合设计原理采用3因素5水平的响应面分析法，研究乙醇浓度、超声时间、液固比及其交互作用对五味子3种主要木脂素提取率的影响。应用SAS软件和响应面分析相结合的方法，模拟得到回归方程的预测模型和可信度，并通过岭脊分析得到最佳的提取条件。结果五味子木脂素超声辅助乙醇提取最佳条件为：乙醇浓度85%、超声时间34 min、液固比146 mL/g。该条件下3种主要木脂素类成分总提取率为1.13%。结论研究建立的模型适合五味子木脂素的提取，可用于实际生产。
목적：우화오미자목지소초성보조제취공예，획취공예삼수。방법이용향응면법(RSM)대오미자목지소초성보조을순제취공예진행우화。재단인소실험기출상，선택을순농도、초성제취시간화액고비위자변량，목지소제취솔위향응치，근거중심조합설계원리채용3인소5수평적향응면분석법，연구을순농도、초성시간、액고비급기교호작용대오미자3충주요목지소제취솔적영향。응용SAS연건화향응면분석상결합적방법，모의득도회귀방정적예측모형화가신도，병통과령척분석득도최가적제취조건。결과오미자목지소초성보조을순제취최가조건위：을순농도85%、초성시간34 min、액고비146 mL/g。해조건하3충주요목지소류성분총제취솔위1.13%。결론연구건립적모형괄합오미자목지소적제취，가용우실제생산。
ABSTRACT:Objective To optimize the extraction technique for lignans from Schisandra chinensis and get the process parameter. Methods Using the response surface method (RSM) to optimize the ultrasonic assisted ethanol extraction process of lignans from Schisandra chinensis. On the basis of single factor experiment, the ethanol concentration, extraction time and liquid-solid ratio were selected as the independent variable, lignans extraction rate response, according to the central composite design principles using three factors and five levels of response surface methodology to study the concentration of ethanol, ultrasonic time, liquid-solid ratio and their interactions on three main lignans extraction rate. Combining SAS and response surface analysis method, the reliability and regression equation prediction model were obtained, and the best extraction conditions were obtained by ridge analysis. Results The conditions were listed as follows:ethanol concentration 85%, ultra-sonic extraction time 34 min, the liquid to solid ratio 146 mL/g. Under the optimum conditions, the extraction yield of lignans was 1.13%. Conclusion The model is suitable for the lignans extracted from Schisandra chi-nensis, and can be used for actual production.