CAJ | 학술논문

目的：采用气相色谱/质谱法测定比较3种海参与3种贝类脂肪酸及脂肪醛二甲基缩醛的方法。方法6种原料经 Folch 法提取总脂,用10%硫酸-甲醇溶液对其进行甲酯化反应,通过气相色谱/质谱法对其脂肪酸及脂肪醛二甲基缩醛的组成进行分析。结果10%硫酸-甲醇溶液能有效地对脂肪酸进行甲酯化衍生,同时促使烯醚键断裂,并与甲醇发生缩醛反应生成脂肪醛二甲基缩醛。通过气相色谱/质谱分析,从3种海参与3种贝类的气相色谱/质谱图中共鉴定出24种脂肪酸,海参中脂肪酸以C16：0, C16：1(n-7)和C20：4(n-6)为主；贝类中脂肪酸以 C16：0, C20：5(n-3)和 C22：6(n-3)为主；海参与贝类中脂肪醛二甲基缩醛均以C18：0DMA为主。结论海参和贝类中的脂肪酸及脂肪醛二甲基缩醛的组成与含量相差较大。
목적：채용기상색보/질보법측정비교3충해삼여3충패류지방산급지방철이갑기축철적방법。방법6충원료경 Folch 법제취총지,용10%류산-갑순용액대기진행갑지화반응,통과기상색보/질보법대기지방산급지방철이갑기축철적조성진행분석。결과10%류산-갑순용액능유효지대지방산진행갑지화연생,동시촉사희미건단렬,병여갑순발생축철반응생성지방철이갑기축철。통과기상색보/질보분석,종3충해삼여3충패류적기상색보/질보도중공감정출24충지방산,해삼중지방산이C16：0, C16：1(n-7)화C20：4(n-6)위주；패류중지방산이 C16：0, C20：5(n-3)화 C22：6(n-3)위주；해삼여패류중지방철이갑기축철균이C18：0DMA위주。결론해삼화패류중적지방산급지방철이갑기축철적조성여함량상차교대。
ABSTRACT:Objective To carry on a comparative study on determination of fatty acids and dimethyl acet-als (DMAs) compositions in 3 kinds of sea cucumbers and 3 kinds of shellfishes by gas chromatography-mass spectrometry(GC/MS). Methods Folch was used to extract the total fat in the 6 materials, and the total fat was treated with 10%sulfuric acid-methanol solution, to prepare for analyzing by GC/MS. Results 10%sul-furic acid-methanol solution can effectively translate FFA into fatty acid methyl ester, and it can also break the vinyl ether bond, and promote the acetal reaction with methanol to generate DMAs. Through the GC/MS anal-ysis, 24 kinds of fatty acids composition in the materials had been identified, C16:0, C16:1(n-7) and C20:4(n-6) were main fatty acids in sea cucumber, and for the shellfish are C20:5(n-3) and the C22:6(n-3). The DMAs in sea cucumber and shellfish were both C18:0DMA. Conclusion Compositions and content of fatty acid and DMAs in sea cucumber and shellfish were significantly different.