CAJ | 학술논문

麻痹性贝类毒素受体蛋白(Saxiphilin, SAX)是一种单体可溶性蛋白,属于转铁蛋白家族,具有转铁蛋白相似的组织分布但其不与 Fe3+结合,可能是一种转运和隔离内源性的有机分子的载体。SAX 可高特异性地结合石房蛤毒素等多种麻痹性贝类毒素,但不与竞争物河豚毒素结合, SAX广泛分布于牛蛙、节肢动物、鱼类、两栖类动物以及爬行动物中,目前主要有两种方式获取SAX,一是通过动物体内提取,另一种是通过体内基因表达。本文从 SAX 蛋白的理化性质、结构和功能研究、分子进化等方面综述了该受体蛋白的研究进展,并结合作者本身的研究工作,展望了 SAX 受体蛋白建立多种麻痹性贝类毒素快速检测方法所存在的问题及应对策略,揭示了SAX在贝类毒素检测及分析中具有广泛的应用前景。
마비성패류독소수체단백(Saxiphilin, SAX)시일충단체가용성단백,속우전철단백가족,구유전철단백상사적조직분포단기불여 Fe3+결합,가능시일충전운화격리내원성적유궤분자적재체。SAX 가고특이성지결합석방합독소등다충마비성패류독소,단불여경쟁물하돈독소결합, SAX엄범분포우우와、절지동물、어류、량서류동물이급파행동물중,목전주요유량충방식획취SAX,일시통과동물체내제취,령일충시통과체내기인표체。본문종 SAX 단백적이화성질、결구화공능연구、분자진화등방면종술료해수체단백적연구진전,병결합작자본신적연구공작,전망료 SAX 수체단백건립다충마비성패류독소쾌속검측방법소존재적문제급응대책략,게시료SAX재패류독소검측급분석중구유엄범적응용전경。
ABSTRACT:Paralytic shellfish toxin (PSP) receptor protein (Saxiphilin, SAX) which belongs to the transferrin family, and has similar tissue distribution but not binding to Fe3+, is a soluble monomeric protein and a carrier of translocation of organic molecules and isolation of endogenous. SAX has high specificity combination with saxitoxin and other paralytic shellfish poisons with high affinity but not competitive tetrodotoxin, which widely distributed in arthropods, fish, amphibians and reptiles. There’re mainly two ways to obtain SAX, one is to ex-tract by the animal, and the other is to gene express in vivo. In this paper, physical and chemical properties, structure and function, molecular evolution, applications and prospects of this receptor protein were reviewed. Combined with the author’s work, the existing problems of PSP detection and the corresponding strategies were put forward, which induced a wide range of application prospects in the detection and analysis of paralytic shellfish poisons.