CAJ | 학술논문

目的：建立一种简便、灵敏、稳定的整鸡中弯曲菌定量检测方法，并用北京地区市售整鸡样品进行验证。方法分别对37株鸡肉中常污染的背景杂菌、24株弯曲菌菌株进行抗生素敏感性测试，对弯曲菌分离用选择性培养基及抗生素添加剂进行优化和改进；用77份人工定量污染弯曲菌的鸡淋洗液进行添加回收实验，以生长指数评价选择性平板对鸡肉样品中背景杂菌的抑制情况和弯曲菌生长状况影响。结果在改良后的Karmali和Preston选择性平板上，弯曲菌生长状态稳定，并可有效抑制鸡肉样品中常见的背景干扰杂菌的生长繁殖，此方法对鸡肉中弯曲菌检出的灵敏度可达2.5 CFU/g。结论所建方法灵敏度高、操作简便、计数准确， Karmali和Preston平板组合后可有效提高鸡肉中弯曲菌的检出率，适用于禽类食品中弯曲菌的定量检测。
목적：건립일충간편、령민、은정적정계중만곡균정량검측방법，병용북경지구시수정계양품진행험증。방법분별대37주계육중상오염적배경잡균、24주만곡균균주진행항생소민감성측시，대만곡균분리용선택성배양기급항생소첨가제진행우화화개진；용77빈인공정량오염만곡균적계림세액진행첨가회수실험，이생장지수평개선택성평판대계육양품중배경잡균적억제정황화만곡균생장상황영향。결과재개량후적Karmali화Preston선택성평판상，만곡균생장상태은정，병가유효억제계육양품중상견적배경간우잡균적생장번식，차방법대계육중만곡균검출적령민도가체2.5 CFU/g。결론소건방법령민도고、조작간편、계수준학， Karmali화Preston평판조합후가유효제고계육중만곡균적검출솔，괄용우금류식품중만곡균적정량검측。
Objective To establish a simple, sensitive, and stabile quantitative enumeration method for Campylobacter spp. from chicken carcass, and to evaluate it by application in detection of Campylobacter spe-cies from retail chicken in Beijing. Methods Based on the plate counting theory, the method of enumeration for Campylobacter species was developed. A total of 37 strains of indigenous flora and 24 strains of Campylo-bacter were used for antimicrobial susceptibility testing, and the selective culture and antibiotic supplements were optimized. Totally, 77 samples with artificial contamination with Campylobacter were evaluated. Growth index was employed for evaluating the growth of Campylobacter as well as the inhibition of indigenous flora in plates. Results Campylobacter spp. grew well and was not inhibited by any antibiotic in selective plates, but indigenous flora could be inhibited on the modified Karmali and Preston plates. The limit of enumeration for Campylobacter species from chicken was 2.5 CFU/g. Conclusion The method could be applied to detect the Campylobacter species in food specifically, quantitatively and accurately. Preston selective agar in combination with Karmali could improve the detection of Campylobacter species from chicken carcass.