CAJ | 학술논문

目的：研究夏枯草水溶性多糖乙醇分级组分的抗氧化活性。方法采用水提醇沉法得到夏枯草水溶性粗多糖，再经不同体积分数(20%、30%、40%、50%、60%、70%， v/v)的乙醇溶液逐级沉淀，依次得到不同分子量的六个夏枯草多糖组分(依次记为PVLP-1、PVLP-2、PVLP-3、PVLP-4、PVLP-5、PVLP-6)。进一步测定了其中四个组分(PVLP-2、PVLP-3、PVLP-4、PVLP-5)对 DPPH、O2-·和·OH 的清除效率。结果从夏枯草中得到的 PVLP-2、PVLP-3、PVLP-4、PVLP-5四个多糖组分对 DPPH 自由基清除效果最明显，在质量浓度为3.2 mg/mL 时，清除率依次为98.09%、90.05%、88.56%和87.71%。各组分对 O2-·以及·OH 自由基的清除作用相对较差些，除PVLP-4清除·OH自由基的EC50值为13.24 mg/mL外，其余均小于10 mg/mL。四个多糖组分中， PVLP-2的总体抗氧化效果优于其他各个组分。结论乙醇分级可以作为一种简便易行的分离纯化方法，制备具有良好抗氧化效果的水溶性夏枯草多糖。
목적：연구하고초수용성다당을순분급조분적항양화활성。방법채용수제순침법득도하고초수용성조다당，재경불동체적분수(20%、30%、40%、50%、60%、70%， v/v)적을순용액축급침정，의차득도불동분자량적륙개하고초다당조분(의차기위PVLP-1、PVLP-2、PVLP-3、PVLP-4、PVLP-5、PVLP-6)。진일보측정료기중사개조분(PVLP-2、PVLP-3、PVLP-4、PVLP-5)대 DPPH、O2-·화·OH 적청제효솔。결과종하고초중득도적 PVLP-2、PVLP-3、PVLP-4、PVLP-5사개다당조분대 DPPH 자유기청제효과최명현，재질량농도위3.2 mg/mL 시，청제솔의차위98.09%、90.05%、88.56%화87.71%。각조분대 O2-·이급·OH 자유기적청제작용상대교차사，제PVLP-4청제·OH자유기적EC50치위13.24 mg/mL외，기여균소우10 mg/mL。사개다당조분중， PVLP-2적총체항양화효과우우기타각개조분。결론을순분급가이작위일충간편역행적분리순화방법，제비구유량호항양화효과적수용성하고초다당。
Objective To investigate the antioxidant activities of soluble polysaccharides fractionated with ethanol. Methods Water soluble crude polysaccharide was extracted from Prunella vulgaris Linn with hot water and precipitated by ethanol, and subsequently separated by ethanol solutions with different concen-trations (20%, 30%, 40%, 50%, 60%, 70%, v/v) into six fractions (PVLP-1, PVLP-2, PVLP-3, PVLP-4, PVLP-5, PVLP-6) with different molecular weights. The antioxidant activities of four fractions (PVLP-2, PVLP-3, PVLP-4, PVLP-5) were tested by determining scavenging rates of the fractions on DPPH, O2-· and ·OH in vitro. Results The four fractions showed obvious antioxidant activities to DPPH radicals. Their scavenging rates were 98.09%, 90.05%, 88.56%and 87.71%at a mass concentration of 3.2 mg/mL, re-spectively. The four fractions showed relatively lower scavenging rates to O2-· and ·OH radicals. The EC50 val-ues in the study were less than 10 mg/mL except for that of PVLP-4 for ·OH free radicals, which was 13.24 mg/mL. Furthermore, PVLP-2 exhibited the strongest antioxidant activities among all the fractions. Conclusion The current study suggest that ethanol solutions with different concentrations would be a good method to iso-late water soluble polysaccharides from Prunella vulgaris Linn bearing excellent antioxidant activities.