解放军医药杂志
解放軍醫藥雜誌
해방군의약잡지
MEDICAL&PHARMACEUTICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY
2014年
6期
81-84
,共4页
姚宝林%杨丽芸%赵静%陈三班%宋跃朋
姚寶林%楊麗蕓%趙靜%陳三班%宋躍朋
요보림%양려예%조정%진삼반%송약붕
补气活血方%支气管炎,慢性%肺气虚%细胞凋亡
補氣活血方%支氣管炎,慢性%肺氣虛%細胞凋亡
보기활혈방%지기관염,만성%폐기허%세포조망
Buqi huoxue decoction%Bronchitis,chronic%Lung-qi deficiency%Apoptosis
目的:探讨补气活血方对慢性支气管炎肺气虚证模型大鼠肺组织细胞凋亡的影响。方法50只SD大鼠随机选取10只作为空白对照组,其余采用改良烟熏法复制慢性支气管炎肺气虚证大鼠模型,造模后按随机数字表法分为模型组、补气活血方高剂量组、补气活血方低剂量组、补肺汤组,每组10只。空白对照组和模型组灌服生理盐水,其余3组分别应用高、低剂量补气活血方和补肺汤进行灌胃干预。采用TUNEL法和流式细胞仪观察肺组织细胞凋亡情况。结果模型组大鼠肺组织炎性细胞凋亡率低于空白对照组,肺实质细胞凋亡率高于空白对照组(P<0.05);补气活血方高、低剂量组和补肺汤组肺组织炎性细胞凋亡率高于模型组,且补气活血方高剂量组高于低剂量组和补肺汤组(P<0.01);补气活血方高、低剂量组和补肺汤组肺组织实质细胞凋亡率低于模型组,且补气活血方高剂量组和低剂量组均低于补肺汤组(P<0.01,P<0.05)。结论补气活血方能够改善慢性支气管炎肺气虚证大鼠肺组织的形态,控制炎症及加强肺组织的自我修复,其机制与诱导肺组织内炎性细胞的凋亡,并抑制肺组织实质细胞的凋亡有关。
目的:探討補氣活血方對慢性支氣管炎肺氣虛證模型大鼠肺組織細胞凋亡的影響。方法50隻SD大鼠隨機選取10隻作為空白對照組,其餘採用改良煙熏法複製慢性支氣管炎肺氣虛證大鼠模型,造模後按隨機數字錶法分為模型組、補氣活血方高劑量組、補氣活血方低劑量組、補肺湯組,每組10隻。空白對照組和模型組灌服生理鹽水,其餘3組分彆應用高、低劑量補氣活血方和補肺湯進行灌胃榦預。採用TUNEL法和流式細胞儀觀察肺組織細胞凋亡情況。結果模型組大鼠肺組織炎性細胞凋亡率低于空白對照組,肺實質細胞凋亡率高于空白對照組(P<0.05);補氣活血方高、低劑量組和補肺湯組肺組織炎性細胞凋亡率高于模型組,且補氣活血方高劑量組高于低劑量組和補肺湯組(P<0.01);補氣活血方高、低劑量組和補肺湯組肺組織實質細胞凋亡率低于模型組,且補氣活血方高劑量組和低劑量組均低于補肺湯組(P<0.01,P<0.05)。結論補氣活血方能夠改善慢性支氣管炎肺氣虛證大鼠肺組織的形態,控製炎癥及加彊肺組織的自我脩複,其機製與誘導肺組織內炎性細胞的凋亡,併抑製肺組織實質細胞的凋亡有關。
목적:탐토보기활혈방대만성지기관염폐기허증모형대서폐조직세포조망적영향。방법50지SD대서수궤선취10지작위공백대조조,기여채용개량연훈법복제만성지기관염폐기허증대서모형,조모후안수궤수자표법분위모형조、보기활혈방고제량조、보기활혈방저제량조、보폐탕조,매조10지。공백대조조화모형조관복생리염수,기여3조분별응용고、저제량보기활혈방화보폐탕진행관위간예。채용TUNEL법화류식세포의관찰폐조직세포조망정황。결과모형조대서폐조직염성세포조망솔저우공백대조조,폐실질세포조망솔고우공백대조조(P<0.05);보기활혈방고、저제량조화보폐탕조폐조직염성세포조망솔고우모형조,차보기활혈방고제량조고우저제량조화보폐탕조(P<0.01);보기활혈방고、저제량조화보폐탕조폐조직실질세포조망솔저우모형조,차보기활혈방고제량조화저제량조균저우보폐탕조(P<0.01,P<0.05)。결론보기활혈방능구개선만성지기관염폐기허증대서폐조직적형태,공제염증급가강폐조직적자아수복,기궤제여유도폐조직내염성세포적조망,병억제폐조직실질세포적조망유관。
Objective To explore the effect of Buqi huoxue decoction on apoptosis of chronic bronchitis rats with deficiency of lung-qi. Methods Ten rats were randomly selected from 50 SD rats as control group, and models of the de-ficiency of lung-qi of chronic bronchitis ( CB) were established using the improved smoking-fumigated method in the other 40 rats. The successful model rats were randomly divided into model group, high-dose group of Buqi huoxue decoction, low-dose group of Buqi huoxue decoction and tonifying lung recipe group ( n=10 for each group) by random digits table. The control and model groups underwent normal saline irrigation, while the other three groups were treated with high and low doses of Buqi huoxue decoction and tonifying lung recipe. Apoptosis conditions were observed with TUNEL kit and flow cytometry. Results The percentage of inflammatory cells apoptosis in model group was significantly lower compared with that in control group (P<0. 05), and the percentage of lung parenchyma cell apoptosis was significantly higher com-pared with that in control group (P<0. 05);the percentages of inflammatory cells apoptosis in high-dose, low-dose and tonifying lung recipe groups were significantly higher compared with that in model group, and the percentage in high-dose group was significantly higher compared with those in low dose and tonifying lung recipe groups (P<0. 01);the percent-ages of lung parenchyma cell apoptosis in high-dose, low-dose and tonifying lung recipe groups were significantly lower compared with that in model group, and the percentages in high-dose and low-dose groups were significantly lower com-pared with that in tonifying lung recipe group (P<0. 01, P<0. 05). Conclusion The Buqi huoxue decoction can im-prove deficiency of lung qi of pulmonary tissue morphology in chronic bronchitis rats. The mechanisms of controlling in-flammation and strengthening the lung tissue self-repair may be related to the apoptosis induction of inflammatory cells in lung tissue and inhibition of lung parenchyma cell apoptosis.