中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2014年
12期
3276-3278
,共3页
王志学%雷燕%王利军%张晓侠%李汝泓
王誌學%雷燕%王利軍%張曉俠%李汝泓
왕지학%뢰연%왕리군%장효협%리여홍
芬太尼%5-氟尿嘧啶%MCF-7%MTT%流式细胞技术%增殖%细胞周期
芬太尼%5-氟尿嘧啶%MCF-7%MTT%流式細胞技術%增殖%細胞週期
분태니%5-불뇨밀정%MCF-7%MTT%류식세포기술%증식%세포주기
Fentanyl%5-fluouracil%MCF-7%MTT%FCM%Proliferation%Cell cycle
目的:研究芬太尼与5-氟尿嘧啶( FU)联合应用对人乳腺癌细胞( MCF-7)增殖与细胞周期的影响。方法药物作用24、48、72 h后噻唑蓝( MTT)法测定MCF-7乳腺癌细胞的增殖抑制情况,选取48 h,通过流式细胞技术( FCM)检测细胞周期情况。结果增殖抑制情况:芬太尼单独应用时,与未处理组相比,均引起平均增殖抑制率(IR)增加(P<0.05);三种浓度的芬太尼与5-FU联合应用作用48、72 h时,均具有协同作用(P<0.05);平均IR随着其中芬太尼浓度增加依次增加。细胞周期影响:单独应用芬太尼处理时,G0/G1期细胞比例随着芬太尼浓度的增加依次增加(P<0.05),S期比例依次降低(P<0.05),增殖指数(PI)亦依次降低。联合用药时,G0/G1期细胞比例随其中芬太尼浓度的增加依次增加(P<0.05), S期、G2/M期细胞、PI随芬太尼浓度的增加依次降低(P<0.05);三种浓度芬太尼与5-FU联合应用,在对 G2/M期细胞比例和 PI减少方面均具有协同作用(P<0.05)。结论一定浓度的芬太尼、5-FU及联合应用对MCF-7乳腺癌细胞有增殖抑制作用,两药联合应用,既抑制了 G0/G1期到 S期的转化,又抑制了S期向G2/M期的转化,可显示出协同作用。并且对增殖抑制和细胞周期的影响也与芬太尼的浓度有一定的剂量-时间依赖关系。
目的:研究芬太尼與5-氟尿嘧啶( FU)聯閤應用對人乳腺癌細胞( MCF-7)增殖與細胞週期的影響。方法藥物作用24、48、72 h後噻唑藍( MTT)法測定MCF-7乳腺癌細胞的增殖抑製情況,選取48 h,通過流式細胞技術( FCM)檢測細胞週期情況。結果增殖抑製情況:芬太尼單獨應用時,與未處理組相比,均引起平均增殖抑製率(IR)增加(P<0.05);三種濃度的芬太尼與5-FU聯閤應用作用48、72 h時,均具有協同作用(P<0.05);平均IR隨著其中芬太尼濃度增加依次增加。細胞週期影響:單獨應用芬太尼處理時,G0/G1期細胞比例隨著芬太尼濃度的增加依次增加(P<0.05),S期比例依次降低(P<0.05),增殖指數(PI)亦依次降低。聯閤用藥時,G0/G1期細胞比例隨其中芬太尼濃度的增加依次增加(P<0.05), S期、G2/M期細胞、PI隨芬太尼濃度的增加依次降低(P<0.05);三種濃度芬太尼與5-FU聯閤應用,在對 G2/M期細胞比例和 PI減少方麵均具有協同作用(P<0.05)。結論一定濃度的芬太尼、5-FU及聯閤應用對MCF-7乳腺癌細胞有增殖抑製作用,兩藥聯閤應用,既抑製瞭 G0/G1期到 S期的轉化,又抑製瞭S期嚮G2/M期的轉化,可顯示齣協同作用。併且對增殖抑製和細胞週期的影響也與芬太尼的濃度有一定的劑量-時間依賴關繫。
목적:연구분태니여5-불뇨밀정( FU)연합응용대인유선암세포( MCF-7)증식여세포주기적영향。방법약물작용24、48、72 h후새서람( MTT)법측정MCF-7유선암세포적증식억제정황,선취48 h,통과류식세포기술( FCM)검측세포주기정황。결과증식억제정황:분태니단독응용시,여미처리조상비,균인기평균증식억제솔(IR)증가(P<0.05);삼충농도적분태니여5-FU연합응용작용48、72 h시,균구유협동작용(P<0.05);평균IR수착기중분태니농도증가의차증가。세포주기영향:단독응용분태니처리시,G0/G1기세포비례수착분태니농도적증가의차증가(P<0.05),S기비례의차강저(P<0.05),증식지수(PI)역의차강저。연합용약시,G0/G1기세포비례수기중분태니농도적증가의차증가(P<0.05), S기、G2/M기세포、PI수분태니농도적증가의차강저(P<0.05);삼충농도분태니여5-FU연합응용,재대 G2/M기세포비례화 PI감소방면균구유협동작용(P<0.05)。결론일정농도적분태니、5-FU급연합응용대MCF-7유선암세포유증식억제작용,량약연합응용,기억제료 G0/G1기도 S기적전화,우억제료S기향G2/M기적전화,가현시출협동작용。병차대증식억제화세포주기적영향야여분태니적농도유일정적제량-시간의뢰관계。
Objective To investigate the effects of different concentration of fentanyl along with 5-FU on the proliferation and cell cycle in MCF-7 breast cancer cells.Methods MTT was applied for evaluation of the antiproliferative inhibitive effects 24, 48 and 72 h after incubation.Moreover, the effect of cell cycle was evaluated by flow cytometry (FCM) 48 h after incubation.Results When using fentanyl alone, the average IR was increased significantly in all three groups (P<0.05), compared with that of untreated group .When using fentanyl combined with 5-FU, the significant synergy was observed in all three groups 48 h or 72 h after incubation(P<0.05).Whereas the IR was positively correlated with the concentration of fentanyl .When using fentanyl alone , along with increasing of the concentration of fentanyl , the rate of G0/G1 phase cell was increased (P<0.05), the rate of S phase cell was decreased (P<0.05), and the PI reduction was also calculat-ed(P<0.05).When using fentanyl combined with 5-FU, the rate of G0/G1 phase cell was increased(P<0.05), nevertheless,the rate of S&G2M phase cells was decreased, as well as PI (P<0.05).Whereas, significant synergy was revealed in all three groups , for the reduc-tion of G2M phase cells rat and PI (P<0.05).Conclusions Fentanyl in certain concentration , 5-FU and the combination of the two have antiproliferative effect on MCF-7 breast cancer cells .Using fentanyl combined with 5-FU, the synergisms is related with the G 0/G1 blockage by morphine and the S blockage by 5-FU, which is decreased the rate of cells in G 2/M phase.In addition, the dose-and time-dependent manners is also shown in a certain extent .