CAJ | 학술논문

目的：识别牛带绦虫新基因,分析和预测该基因及其编码蛋白的结构及特性,为其生物学功能研究提供依据。方法利用生物信息学在线分析网站、工具进行序列分析、预测其编码的膜联蛋白B3的理化特性、翻译后的修饰、功能域、亚细胞定位、拓扑结构、二级结构、三维空间构象等。结果该基因全长1259 bp,编码区为106-1074bp,编码322个氨基酸,为全长基因；无跨膜区；蛋白质在溶液中的性质不稳定,理论分子量为36415.1；没有质体、线粒体定位序列。结论运用生物信息学方法从牛带绦虫成虫cDNA文库中识别出了牛带绦虫成虫膜联蛋白B3基因,并对其所编码的蛋白质的结构与功能进行预测分析。
목적：식별우대조충신기인,분석화예측해기인급기편마단백적결구급특성,위기생물학공능연구제공의거。방법이용생물신식학재선분석망참、공구진행서렬분석、예측기편마적막련단백B3적이화특성、번역후적수식、공능역、아세포정위、탁복결구、이급결구、삼유공간구상등。결과해기인전장1259 bp,편마구위106-1074bp,편마322개안기산,위전장기인；무과막구；단백질재용액중적성질불은정,이론분자량위36415.1；몰유질체、선립체정위서렬。결론운용생물신식학방법종우대조충성충cDNA문고중식별출료우대조충성충막련단백B3기인,병대기소편마적단백질적결구여공능진행예측분석。
Objective recognition cattle belt tapeworm new genes, analysis and forecast the gene and its coding protein structure and properties for its biological function provides the basis for the research. Methods using bioinformatics online analysis of site, tools for sequence analysis, forecasting the coded annexin B3 physical and chemical characteristics, antigen epitope, translation modifications, function domain, the subcel ular localization, the topology structure, secondary structure, the three dimensional space conformation, evolutionary tree, etc. Results the gene (1259 bp, coding region for 106-1074 bp, code 322 amino acids, broke ground for the genes; No cross membrane area; Protein in solution of the nature is not stable, theory molecular weight was 36415.1; No plastid, mitochondrial positioning sequence. Conclusion using bioinformatics methods from cattle belt tapeworm imago cDNA library used to identify the cows with tapeworm imago annexin B3 gene, and the encoded protein structure and function of forecasting analysis.