医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2014年
12期
51-52
,共2页
I型PLA2 N-端衍生肽%杀菌活性%构效关系
I型PLA2 N-耑衍生肽%殺菌活性%構效關繫
I형PLA2 N-단연생태%살균활성%구효관계
group I phospholipase A2%N-terminal derived peptide%bactericidal activity%structure- activity relationship
目的：研究人、猩猩、猪、小鼠、大鼠五种生物I型磷脂酶A2（phospholipase A2, PLA2）N末端衍生肽在体外的杀菌效应，了解它们的结构与功能的关系。方法：根据人、猩猩、猪、小鼠、大鼠五种生物I型PLA2N-端第1-15个氨基酸残基为模板，分别合成衍生多肽AVW、ALW、AVN（因人与黑猩猩、大鼠与小鼠I型P L A2N-端第1-15个氨基酸残基序列相同，最终合成3条衍生多肽）。采用琼脂铺板计数法，将不同浓度的多肽分别与革兰阳性菌金黄色葡萄球菌和革兰阴性菌大肠杆菌在37℃孵育2.5h，然后铺板并置于37℃恒温箱培养18～24h，记录每一块琼脂板上的菌落形成数，并计算多肽杀菌率。结果：衍生多肽A V W、ALW、AVN对革兰阳性菌金黄色葡萄球菌和革兰阴性菌大肠杆菌均有杀菌活性，AVW和AVN对金黄色葡萄球菌和大肠杆菌杀菌作用较强，ALW对G+菌及G-菌杀菌活性较弱。结论：不同生物I型PLA2N-末端衍生肽对G+菌和G-菌均有杀菌活性，而具有较多碱性氨基酸衍生肽杀菌活性较强。
目的：研究人、猩猩、豬、小鼠、大鼠五種生物I型燐脂酶A2（phospholipase A2, PLA2）N末耑衍生肽在體外的殺菌效應，瞭解它們的結構與功能的關繫。方法：根據人、猩猩、豬、小鼠、大鼠五種生物I型PLA2N-耑第1-15箇氨基痠殘基為模闆，分彆閤成衍生多肽AVW、ALW、AVN（因人與黑猩猩、大鼠與小鼠I型P L A2N-耑第1-15箇氨基痠殘基序列相同，最終閤成3條衍生多肽）。採用瓊脂鋪闆計數法，將不同濃度的多肽分彆與革蘭暘性菌金黃色葡萄毬菌和革蘭陰性菌大腸桿菌在37℃孵育2.5h，然後鋪闆併置于37℃恆溫箱培養18～24h，記錄每一塊瓊脂闆上的菌落形成數，併計算多肽殺菌率。結果：衍生多肽A V W、ALW、AVN對革蘭暘性菌金黃色葡萄毬菌和革蘭陰性菌大腸桿菌均有殺菌活性，AVW和AVN對金黃色葡萄毬菌和大腸桿菌殺菌作用較彊，ALW對G+菌及G-菌殺菌活性較弱。結論：不同生物I型PLA2N-末耑衍生肽對G+菌和G-菌均有殺菌活性，而具有較多堿性氨基痠衍生肽殺菌活性較彊。
목적：연구인、성성、저、소서、대서오충생물I형린지매A2（phospholipase A2, PLA2）N말단연생태재체외적살균효응，료해타문적결구여공능적관계。방법：근거인、성성、저、소서、대서오충생물I형PLA2N-단제1-15개안기산잔기위모판，분별합성연생다태AVW、ALW、AVN（인인여흑성성、대서여소서I형P L A2N-단제1-15개안기산잔기서렬상동，최종합성3조연생다태）。채용경지포판계수법，장불동농도적다태분별여혁란양성균금황색포도구균화혁란음성균대장간균재37℃부육2.5h，연후포판병치우37℃항온상배양18～24h，기록매일괴경지판상적균락형성수，병계산다태살균솔。결과：연생다태A V W、ALW、AVN대혁란양성균금황색포도구균화혁란음성균대장간균균유살균활성，AVW화AVN대금황색포도구균화대장간균살균작용교강，ALW대G+균급G-균살균활성교약。결론：불동생물I형PLA2N-말단연생태대G+균화G-균균유살균활성，이구유교다감성안기산연생태살균활성교강。
Object:To analysis and compare the bactericidal activity of the polypeptides derives from the N-terminal sequence of group I phospholipase A2 in Homo sapiens、Pan troglodytes、Sus scrofa、Rattus norvegicus and Mus musculus in vitro, to understand the relationship between the bactericidal activity of the derived peptide and molecular structure. Methods:According to the N-terminal 15 amino acid sequence of group I PLA2 on the five mammal , The AVW, ALW and AVN were synthesized (because of the sequence of group I PLA2 from the Homo sapiens and the Pan troglodytes,the Rattus norvegicus and the Mus musculus were the same ,so only 3 polypeptides were synthesized). Different concentrations of peptide incubated with Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli) in a water bath at 37℃ for 2.5 h. The reaction solution mixed with agar solution and poured plates. The plates were incubated at a temperature of 37℃ for 18-24 h, then were took out to count the colony-forming units (CFU) of each agar plate. The bactericidal rate was calculated .Results:3 peptides derived from the N-terminal sequence of group I phospholipase A2 on mammal possessed bactericidal activity on the Staphylococcus aureus and the Escherichia coli. The bactericidal activity of AVW and AVN were stronger than ALW on Staphylococcus aureus and Escherichia coli. Conclusion: Peptides of the AVW, ALW and AVN, which derived from group I PLA2 N-terminal sequence possess a bactericidal activity. The bactericidal potency may be related to the different structure The peptide which carried more basic amino acid residues possesses stronger bactericidal activity.
