CAJ | 학술논문

建立了反相高效液相色谱法测定金银忍冬叶、花和果实中的绿原酸含量的测定方法。利用超声波辅助提取金银忍冬叶、花和果实中的绿原酸，采用采用反相高效液相色谱法测定金银忍冬叶、花和果实中的绿原酸含量。结果表明：在流动相为甲醇（A）：1.0％的冰醋酸溶液（B）＝27：73，色谱柱为Alltima C18 Column （250 mm ×4.6 mm，5μm），流动相流速为1.0 mL／min，检测波长为327 nm，柱温为25℃的实验条件下测定金银忍冬叶、花和果实中的绿原酸含量分别为：（31.64±2.21） mg／g，（17.45±1.05） mg／g，（5.14±1.39） mg／g，且宁夏金银忍冬植株不同部位中绿原酸类化合物的含量分布为：叶＞花＞果实。回收率为99.74％， RSD为0.23％。所建立的方法稳定，操作简单，结果可靠。
건립료반상고효액상색보법측정금은인동협、화화과실중적록원산함량적측정방법。이용초성파보조제취금은인동협、화화과실중적록원산，채용채용반상고효액상색보법측정금은인동협、화화과실중적록원산함량。결과표명：재류동상위갑순（A）：1.0％적빙작산용액（B）＝27：73，색보주위Alltima C18 Column （250 mm ×4.6 mm，5μm），류동상류속위1.0 mL／min，검측파장위327 nm，주온위25℃적실험조건하측정금은인동협、화화과실중적록원산함량분별위：（31.64±2.21） mg／g，（17.45±1.05） mg／g，（5.14±1.39） mg／g，차저하금은인동식주불동부위중록원산류화합물적함량분포위：협＞화＞과실。회수솔위99.74％， RSD위0.23％。소건립적방법은정，조작간단，결과가고。
Chlorogenic acid in the leaves, flowers and fruits of Lonicera maackii were simultaneously determined by a high performance liquid chromatography method.The samples were separated by a Alltima C18 column using methanol and 1.0% acetic acid as a mixed mobile phase.The detection wavelength was 327 nm.The analytical method displayed a wide linear range in 2~8 μg/mL for the chlorogenic acid.The contents of total chlorogenic acid were (31.64 ±0.07) mg/g and (17.45 ±0.06) mg/g, and(5.14 ±0.27) mg/g in the leaves, flowers and fruits of Lonicera maackii of Ningxia province, respectively.The average recovery was 99.74% and RSD was 0.23%.This method was simple, rapid, accurate and sensitive, and Lonicera maackii of Ningxia was a good source of chlorogenic acids.