医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2014年
14期
87-88
,共2页
山豆根提取物%苦参碱%质量研究%高效液相色谱法
山豆根提取物%苦參堿%質量研究%高效液相色譜法
산두근제취물%고삼감%질량연구%고효액상색보법
Sophorae Tonkinensis Radix Et Rhizoma Extracts%Matrine%quality study%HPLC
目的：优选山豆根药材的提取工艺。方法：以提取物中苦参碱的含量为研究指标，采用高效液相色谱法测定其含量；比较水提醇沉法、60℃酸水浸泡醇沉，pH8-9氨水提取醇沉法、60%乙醇加热回流法等不同工艺的差异。结果：60%乙醇加热回流法苦参碱含量较高，苦参碱含量在6.24μg～399.04μg范围里呈较好的线性关系；苦参碱的回收率为100.08%。结论：60%乙醇加热回流法，提取山豆根药材的提取物中苦参碱含量较高，苦参碱转移率最高，提取方法合理、操作简单，适合用于大生产提取工艺。
目的：優選山豆根藥材的提取工藝。方法：以提取物中苦參堿的含量為研究指標，採用高效液相色譜法測定其含量；比較水提醇沉法、60℃痠水浸泡醇沉，pH8-9氨水提取醇沉法、60%乙醇加熱迴流法等不同工藝的差異。結果：60%乙醇加熱迴流法苦參堿含量較高，苦參堿含量在6.24μg～399.04μg範圍裏呈較好的線性關繫；苦參堿的迴收率為100.08%。結論：60%乙醇加熱迴流法，提取山豆根藥材的提取物中苦參堿含量較高，苦參堿轉移率最高，提取方法閤理、操作簡單，適閤用于大生產提取工藝。
목적：우선산두근약재적제취공예。방법：이제취물중고삼감적함량위연구지표，채용고효액상색보법측정기함량；비교수제순침법、60℃산수침포순침，pH8-9안수제취순침법、60%을순가열회류법등불동공예적차이。결과：60%을순가열회류법고삼감함량교고，고삼감함량재6.24μg～399.04μg범위리정교호적선성관계；고삼감적회수솔위100.08%。결론：60%을순가열회류법，제취산두근약재적제취물중고삼감함량교고，고삼감전이솔최고，제취방법합리、조작간단，괄합용우대생산제취공예。
Objective: To optimize the extraction technology for the Sophorae Tonkinensis Radix Et Rhizoma. Methods: Taking the content of the Matrine within the extracts as a study index, we use HPLC to determine its content. Then we compare differences among Water extraction - Alcohol precipitation,60℃Acid water soaking- Alcohol precipitation,pH8-9 Ammonia water extraction Alcohol precipitation and 60% Alcohol Heating reflux method.Result: The content of the Matrine from 60% Alcohol Heating reflux method is the higher.In the range of 6.24μg~399.04μg,the content of the Matrine shows a good linear relationship and the recovery is 100.08%.Conclusion: Using the 60% alcohol Heating reflux method, the content of the Matrine from the Sophorae Tonkinensis Radix Et RhizomaExtracts is the higher, with a highest metastasis rate. The extracting method is reasonable, simple and suitable for industrialized production.
