CAJ | 학술논문

目的：本文通过实验考察了AfPGA催化合成头孢克洛的工艺及其产物的分离纯化方法，最终确定最优反应条件为：pH（7.0）；温度（25℃）；缓冲体系（磷酸钠）；酶投入量（3U/mL）；底物浓度（7-ACCA60mmol/L，苯甘氨酸甲酯120mmol/L）。在此条件下底物转化率将达到85%以上，产物纯度可超过99.5%，杂质含量低于0.5%。
목적：본문통과실험고찰료AfPGA최화합성두포극락적공예급기산물적분리순화방법，최종학정최우반응조건위：pH（7.0）；온도（25℃）；완충체계（린산납）；매투입량（3U/mL）；저물농도（7-ACCA60mmol/L，분감안산갑지120mmol/L）。재차조건하저물전화솔장체도85%이상，산물순도가초과99.5%，잡질함량저우0.5%。
this article through the experiment investigates the AfPGA catalytic synthesis of cefaclor process and product separation and purification method, final y the optimal reaction conditions were determined as: pH (7.0); Temperature (25℃); (sodium phosphate buffer system); Enzyme mass (3u/mL); The substrate concentration (7-ACCA60mmol/L, methyl phenyl glycine tendency for 120/L). Under the condition of the substrate conversion rate wil reach more than 85%, product purity over 99.5%, impurity content lower than 0.5%.