CAJ | 학술논문

目的探讨模拟不同海拔高原环境下氦氧潜水对潜水员血细胞的影响.方法 4名潜水员于高、低压两用舱内,连续9d先后模拟平原、海拔3000 m环境及该环境下30 m潜水、海拔4000m环境及该环境下30 m潜水、海拔5200 m环境及该环境下30 m潜水和50 m潜水.分别于实验第1天早上进舱前空腹；实验第3天3000 m高原早上空腹；实验第4天3000 m高原30 m潜水减压返回3000 m后,当晚17:30晚饭前；实验第6天4000 m高原30 m潜水减压返回4000 m后,当晚17:30晚饭前；实验第7天5200 m高原停留1晚,早上空腹；实验第8天5200 m高原50 m潜水减压回到5200 m后,晚23:00;实验第9天实验结束后出舱午餐前抽血.采用自动血细胞计数仪进行血常规检查,包括:白细胞计数、白细胞分类、红细胞计数、血红蛋白、红细胞压积、血小板计数；采用流式细胞仪进行T淋巴细胞亚群检测.结果从实验开始至实验结束出舱后的各个时间点,白细胞有逐渐升高的趋势,但差异无统计学意义(P＞0.05)；在海拔5200 m[(6.21 ±3.27)×109/L]高原环境停留时,中性粒细胞百分比较平原[(3.22±1.18)×109/L]及海拔3000 m[(3.43 ±1.18)×109/L]高原明显升高(P＜0.01),而淋巴细胞百分比则明显下降(P＜0.01),中性粒细胞及淋巴细胞计数亦有相应的改变(P＜0.05)；在海拔5200 m高原环境50 m潜水减压回到5200 m时[(2.80±0.30)×109/L],淋巴细胞计数较平原[(2.02±0.15)×109/L]及海拔3000 m高原[(2.20 ±0.26)×109/L]明显升高(P＜0.05)；其余时间点白细胞变化不明显.实验过程中各时间点红细胞、血红蛋白、红细胞压积、血小板计数及淋巴细胞亚群变化较平原及海拔3000 m高原差异无统计学意义(P＞0.05).结论本次实验条件下的模拟高原氦氧潜水过程对潜水员的血常规及T淋巴细胞没有造成明显的影响.
목적 탐토모의불동해발고원배경하양양잠수대잠수원혈세포적영향.방법 4명잠수원우고、저압량용창내,련속9d선후모의평원、해발3000 m배경급해배경하30 m잠수、해발4000m배경급해배경하30 m잠수、해발5200 m배경급해배경하30 m잠수화50 m잠수.분별우실험제1천조상진창전공복；실험제3천3000 m고원조상공복；실험제4천3000 m고원30 m잠수감압반회3000 m후,당만17:30만반전；실험제6천4000 m고원30 m잠수감압반회4000 m후,당만17:30만반전；실험제7천5200 m고원정류1만,조상공복；실험제8천5200 m고원50 m잠수감압회도5200 m후,만23:00;실험제9천실험결속후출창오찬전추혈.채용자동혈세포계수의진행혈상규검사,포괄:백세포계수、백세포분류、홍세포계수、혈홍단백、홍세포압적、혈소판계수；채용류식세포의진행T림파세포아군검측.결과 종실험개시지실험결속출창후적각개시간점,백세포유축점승고적추세,단차이무통계학의의(P＞0.05)；재해발5200 m[(6.21 ±3.27)×109/L]고원배경정류시,중성립세포백분비교평원[(3.22±1.18)×109/L]급해발3000 m[(3.43 ±1.18)×109/L]고원명현승고(P＜0.01),이림파세포백분비칙명현하강(P＜0.01),중성립세포급림파세포계수역유상응적개변(P＜0.05)；재해발5200 m고원배경50 m잠수감압회도5200 m시[(2.80±0.30)×109/L],림파세포계수교평원[(2.02±0.15)×109/L]급해발3000 m고원[(2.20 ±0.26)×109/L]명현승고(P＜0.05)；기여시간점백세포변화불명현.실험과정중각시간점홍세포、혈홍단백、홍세포압적、혈소판계수급림파세포아군변화교평원급해발3000 m고원차이무통계학의의(P＞0.05).결론 본차실험조건하적모의고원양양잠수과정대잠수원적혈상규급T림파세포몰유조성명현적영향.
Objective To investigate effects of simulated heliox diving on blood cells of the divers at different high altitudes.Methods In the dual-purpose hyperbaric and hypobaric chamber complex,4 divers conducted a series of simulated dives for a succession of 9 days at various altitudes and under different simulated environmental conditions:the 30 m dive at an altitude of 3000 m,the 30 m dive at an altitude of 4000 m,and the 30 m and 50 m dives at an altitude of 5200 m.Blood samples were taken respectively at the following time points:on the morning of the first day of the experiment before they entered the chamber on an empty stomach;on the morning of the third day of the experiment at an altitude of 3000 m also on an empty stomach; at 17:30 pm before supper on the fourth day of the experiment,when the divers were decompressed and returned to the surface at an altitude of 3000 m,upon completion of the 30 m-dive; at 17:30 pm before supper on the sixth day,when the divers returned to the surface at an altitude of 4000 m,upon completion of the 30 m-dive; on the morning of the seventh day on an empty stomach,the divers stayed overnight at an altitude of 5200 m; at 23:00 on the eighth day,when the divers were decompressed to an altitude of 5200 m,upon completion of the 50 m-dive; before lunch on the ninth day,when the divers came out of the chamber upon completion of the experiment.Blood components including WBC,WBC classification,RBC,hemoglobin,lymphocyte,granulocyte,hemoglobin,hematocrit,platelet and T lymphocyte subpopulation,were measured with flow cytometry.Results White blood cells detected at various time points both before and after the experiment tended to increase gradually,but without statistical significance (P ＞ 0.05).When the divers stayed at the height of 5200 m above sea level,the percentage of granulocyte was significantly higher [(6.21 ± 3.27) × 109/L] than those at the sea level [(3.22 ± 1.18) × 109/L] and at 3000 m above sea level [(3.43 ± 1.18) × 109/L] (P ＜ 0.01),while the percentage of lymphocyte decreased significantly (P ＜ 0.01) and corresponding changes in the amount of granulocyte and lymphocyte could be observed at the same time points (P ＜ 0.05).When the divers were decompressed to the altitude of 5200 m upon completion of the 50 m simulated dive at 5200 m above sea level,lymphocyte counts significantly increased [(2.28 ± 0.30) × 109/L],when compared with those at the plain [(2.02 ±0.15) × 109/L] and at 3000 m[(2.20 ±0.26) × 109/L] (P ＜ 0.05).No obvious changes could be noted in white cells at other time points.No statistical significance could be noticed in changes of red blood cell,hemoglobin,hematocrit,platelet and T lymphocyte subpopulation at various time points during the experiment,when compared with those at the plain and at 3000 m (P ＞ 0.05).Conclusions The simulated heliox diving at different high altitudes didnt seemed to have obvious effects on the blood routines of the divers.