CAJ | 학술논문

文章根据已知植物eEF-1a（编码 Eukaryotic elongation factor1-alpha）基因的保守序列设计引物，采用 RT- PCR的方法扩增独行菜（Lepidium apetalum Willd．）eEF-1a基因片段。结果获得一大小为570bp 的基因片段，序列比对表明，该基因片段与拟南芥eEF-1a基因核苷酸序列的同源性达到95％，推测该其应该是独行菜eEF-1a基因片段。采用RT- PCR方法，对独行菜三个生长阶段材料及对应阶段冷诱导后地材料中的eEF-1a基因表达作了分析，表明eEF-1a在独行菜组织中表达稳定，可以作为实时荧光定量 PCR研究独行菜基因表达分析中的看家基因。
문장근거이지식물eEF-1a（편마 Eukaryotic elongation factor1-alpha）기인적보수서렬설계인물，채용 RT- PCR적방법확증독행채（Lepidium apetalum Willd．）eEF-1a기인편단。결과획득일대소위570bp 적기인편단，서렬비대표명，해기인편단여의남개eEF-1a기인핵감산서렬적동원성체도95％，추측해기응해시독행채eEF-1a기인편단。채용RT- PCR방법，대독행채삼개생장계단재료급대응계단랭유도후지재료중적eEF-1a기인표체작료분석，표명eEF-1a재독행채조직중표체은정，가이작위실시형광정량 PCR연구독행채기인표체분석중적간가기인。
Degenerate primers were designed based on the conserved sequences of the eEF-1a genes from oth-er plants. And the eEF-1a gene fragment was obtained by RT- PCR from Lepidium (Lepidium apetalum Willd.). The eEF-1a gene fragment contained 570 bp nucleotide acids. Blasting analysis showed that it has 95% nucleotide sequence homology with one in arabidopsis thaliana. And RT-PCR analysis showed that the expression of eEF-1a gene was steady in Lepidium, and it can be used as internal standard when study on other genes’ expression and regulation in Lepidium.