CAJ | 학술논문

该文从活性污泥中分离出三株高效的异养硝化好氧反硝化菌。这些菌经形态特征和生理生化特征分析以及16S rRNA鉴定，命名为B． subtilis CL1、B． subtilis CL9和G． terrae CL6。在以柠檬酸钠为碳源、硫酸铵为氮源、初始氨氮浓度为50 mg/L、碳氮比为20、温度为30℃、pH为7、反应时间为48 h的条件下，B． subtilis CL1和B． subtilis CL9对氨氮的去除率均为99％，对总氮的去除率分别为98％和96％。以乙酸钠为碳源，其他条件与上述两种菌相同的条件下，G． terrae CL6对氨氮和总氮的去除率均达到100％，且在硝化过程中没有亚硝酸盐氮或硝酸盐氮的累积。
해문종활성오니중분리출삼주고효적이양초화호양반초화균。저사균경형태특정화생리생화특정분석이급16S rRNA감정，명명위B． subtilis CL1、B． subtilis CL9화G． terrae CL6。재이저몽산납위탄원、류산안위담원、초시안담농도위50 mg/L、탄담비위20、온도위30℃、pH위7、반응시간위48 h적조건하，B． subtilis CL1화B． subtilis CL9대안담적거제솔균위99％，대총담적거제솔분별위98％화96％。이을산납위탄원，기타조건여상술량충균상동적조건하，G． terrae CL6대안담화총담적거제솔균체도100％，차재초화과정중몰유아초산염담혹초산염담적루적。
3 efficient heterotrophic nitrification and aerobic denitrification bacteria were isolated and named as Bacillus subtilis CL1, Bacillus subtilis CL9 and Gordonia terrae CL6 according to their morphological,physiological and biochemical characters,and 16S rRNA identification. When sodium citrate was used as carbon source,ammonium sulfate was used as nitrogen,initial ammonia concentration was 50 mg/L,carbon/ammonia ratio was 20,temperature was 30 ℃,pH was 7 and reaction time was 48 h,removal rates of ammonia and total nitrogen by B. subtilis CL1 were 99% and 98%,respectively,and those of by B. subtilis CL9 were 99%and 96%,respectively. Removal rates of both ammonia and total nitrogen by G. terrae CL6 were 100% on the almost same conditions as above except for using sodium acetate as carbon source and neither nitrate nor nitrite were detected during the nitrification process.