食品与发酵科技
食品與髮酵科技
식품여발효과기
SICHUAN FOOD AND FERMENTATION
2014年
3期
1-4,11
,共5页
张波%杨静云%刘玉川%孟涛%李学如
張波%楊靜雲%劉玉川%孟濤%李學如
장파%양정운%류옥천%맹도%리학여
ε-聚赖氨酸%不吸水链霉菌%诱变育种
ε-聚賴氨痠%不吸水鏈黴菌%誘變育種
ε-취뢰안산%불흡수련매균%유변육충
ε-polylysine%Streptomyces ahygroscopicus%mutation breeding
采用Nishikawa法，从成都郊区土壤中筛选获得一株ε-聚赖氨酸（ε-PL）产量为1.893g/L的菌株SAC23，通过16S rRNA分子鉴定，初步确定该菌株为Streptomyces ahygroscopicus （不吸水链霉菌）。以SAC23为出发菌株，通过60Coγ射线诱变，结合亚甲基蓝与链霉素抗性平板筛选，获得1株遗传性能稳定，ε-PL产量可达4.384g/L的变异菌株SAS17，与SAC23相比较，ε-PL产量提高了131.6%。表明通过60Coγ射线诱变，结合亚甲基蓝与链霉素抗性平板筛选可大幅提高链霉菌生产ε-聚赖氨酸的能力。
採用Nishikawa法，從成都郊區土壤中篩選穫得一株ε-聚賴氨痠（ε-PL）產量為1.893g/L的菌株SAC23，通過16S rRNA分子鑒定，初步確定該菌株為Streptomyces ahygroscopicus （不吸水鏈黴菌）。以SAC23為齣髮菌株，通過60Coγ射線誘變，結閤亞甲基藍與鏈黴素抗性平闆篩選，穫得1株遺傳性能穩定，ε-PL產量可達4.384g/L的變異菌株SAS17，與SAC23相比較，ε-PL產量提高瞭131.6%。錶明通過60Coγ射線誘變，結閤亞甲基藍與鏈黴素抗性平闆篩選可大幅提高鏈黴菌生產ε-聚賴氨痠的能力。
채용Nishikawa법，종성도교구토양중사선획득일주ε-취뢰안산（ε-PL）산량위1.893g/L적균주SAC23，통과16S rRNA분자감정，초보학정해균주위Streptomyces ahygroscopicus （불흡수련매균）。이SAC23위출발균주，통과60Coγ사선유변，결합아갑기람여련매소항성평판사선，획득1주유전성능은정，ε-PL산량가체4.384g/L적변이균주SAS17，여SAC23상비교，ε-PL산량제고료131.6%。표명통과60Coγ사선유변，결합아갑기람여련매소항성평판사선가대폭제고련매균생산ε-취뢰안산적능력。
A ε-polylysine (ε-PL) producer strain SAC23 with 1.893g/L yield, was screened from the soil collected from the suburbs of Chengdu, by using Nishikawa's method. The phylogenetic tree based on the 16S rRNA se-quences indicates that it belongs to Streptomyces ahygroscopic. A mutant SAS17 with high-production of ε-PL and stable genetic performance was screened by 60Co ray combining drug-resistance technique and methylene blue plate. The production of ε-PL reached to 4.384g/L, increasing 131.6% compared with that of the original strain SAC23. It shows that the production of ε-polylysine of ahygroscopicus can be improved significantly through the method of 60Co mutagenesis combined with streptomycin resistance plate.
