CAJ | 학술논문

【目的】建立从棒柄花（Cleidion brevipetiolatum Paxet Hoffm）中制备棒柄花苷 A（PPGA）对照品的方法。【方法】利用硅胶柱层析、重结晶和反相制备高效液相色谱对棒柄花的乙醇提取物进行分离纯化，以 HPLC对棒柄花苷 A进行纯度检查和含量测定，并通过 UV，IR，MS，1 HNMR等对其进行结构确证。【结果】从棒柄花叶中分离、纯化出棒柄花苷 A对照品，质量分数W＞98．0％。【结论】所建立的制备方法简单，制备出的棒柄花苷 A对照品符合中药化学对照品的相关要求，可作为棒柄花药材和含棒柄花成药质量控制，以及中药药效物质基础用的化学对照品。
【목적】건립종봉병화（Cleidion brevipetiolatum Paxet Hoffm）중제비봉병화감 A（PPGA）대조품적방법。【방법】이용규효주층석、중결정화반상제비고효액상색보대봉병화적을순제취물진행분리순화，이 HPLC대봉병화감 A진행순도검사화함량측정，병통과 UV，IR，MS，1 HNMR등대기진행결구학증。【결과】종봉병화협중분리、순화출봉병화감 A대조품，질량분수W＞98．0％。【결론】소건립적제비방법간단，제비출적봉병화감 A대조품부합중약화학대조품적상관요구，가작위봉병화약재화함봉병화성약질량공제，이급중약약효물질기출용적화학대조품。
Objective]A seperation method was established to prepare reference substance of PP-GA from leave of Cleidion brevipetiolatum Paxet Hoffm.[Methods]Ethanol-extract of the leave of Cleidion brevipetiolatum Paxet Hoffm was isolated and purified by RP-HPLC combi-ning with column chromatography and recrystallization.Its structure was identified by UV,IR, MS,1 HNMR.[Results]PPGA was completely seperated from leave ofCleidion brevipetiolatum Paxet Hoffm.The purity of the reference substance was not less than 98.0%.[Conclusion]The method is simple and convenient,by which the prepared compound is accorded with the relative standards of chemical reference substance in Chinese materia medica.It can be used as a refer-ence subsatance for the quality control and the research of herbal medicine.