CAJ | 학술논문

目的：确认PowerPlex?21试剂盒与GoldeneyeTM 20A试剂盒分型结果的一致性。方法应用两试剂盒对205名北京汉族无关个体血样DNA进行复合扩增，观察19个重叠STR基因座分型的一致性，并统计D1S1656的遗传多态性。结果所有19个重叠基因座分型相同，两个试剂盒的杂合基因座峰高比例差异无统计学意义（P>0.05）。D1S1656杂合度为0.878，个人识别率为0.949，三联体非父排除率为0.751，二联体非父排除率为0.506，多态信息含量为0.810。结论 PowerPlex?21试剂盒与GoldeneyeTM 20A试剂盒分型结果一致性好，引物设计合理；D1S1656多态性好，可用于人类遗传分析及法医学中的亲子鉴定和个人识别。
목적：학인PowerPlex?21시제합여GoldeneyeTM 20A시제합분형결과적일치성。방법응용량시제합대205명북경한족무관개체혈양DNA진행복합확증，관찰19개중첩STR기인좌분형적일치성，병통계D1S1656적유전다태성。결과소유19개중첩기인좌분형상동，량개시제합적잡합기인좌봉고비례차이무통계학의의（P>0.05）。D1S1656잡합도위0.878，개인식별솔위0.949，삼련체비부배제솔위0.751，이련체비부배제솔위0.506，다태신식함량위0.810。결론 PowerPlex?21시제합여GoldeneyeTM 20A시제합분형결과일치성호，인물설계합리；D1S1656다태성호，가용우인류유전분석급법의학중적친자감정화개인식별。
To ensure the consistency of genotype results for PowerPlex?21 kit and GoldeneyeTM 20A kit. Methods The STR loci were amplified in DNA samples from 205 unrelated individuals in Beijing Han population. And consistency of 19 overlap STR loci typing were observed. The genetic polymorphism of D1S1656 locus was obtained. Results All 19 overlap loci typing showed consistent. The proportion of peak height of heterozygous loci in two kits showed no statistical difference (P>0.05). The observed heterozygosis of D1S1656 was 0.878. The discrimination power was 0.949. The excluding probability of paternity of triplet was 0.751. The excluding probability of paternity of diploid was 0.506. The polymor-phism information content was 0.810. Conclusion PowerPlex?21 kit and GoldeneyeTM 20A kit present a good consistency. The primer design is reasonable. The polymorphism of D1S1656 is good. The two kits can be used for human genetic analysis, paternity test, and individual identification in forensic practice.