CAJ | 학술논문

目的：观察钙-钙调素依赖性蛋白激酶Ⅱδ（calcium-calmodulin dependent protein kinaseⅡδ，CaMKⅡδ）在脑损伤后皮质中不同时段的表达变化。方法建立大鼠脑外伤模型，通过Western印迹法和免疫组织化学染色法检测损伤区周围皮质CaMKⅡδ的表达变化。结果 Western印迹法显示，CaMKⅡδ在正常皮质中有表达，损伤后表达上调，在损伤3d达到高峰，并随着损伤时间延长逐渐恢复到正常水平。免疫组织化学结果与Western印迹法结果一致。结论 CaMKⅡδ在脑组织损伤灶及其周围呈现先增高后降低的表达变化，为法医学推断脑外伤后损伤经过时间提供了新的参考指标。
목적：관찰개-개조소의뢰성단백격매Ⅱδ（calcium-calmodulin dependent protein kinaseⅡδ，CaMKⅡδ）재뇌손상후피질중불동시단적표체변화。방법건립대서뇌외상모형，통과Western인적법화면역조직화학염색법검측손상구주위피질CaMKⅡδ적표체변화。결과 Western인적법현시，CaMKⅡδ재정상피질중유표체，손상후표체상조，재손상3d체도고봉，병수착손상시간연장축점회복도정상수평。면역조직화학결과여Western인적법결과일치。결론 CaMKⅡδ재뇌조직손상조급기주위정현선증고후강저적표체변화，위법의학추단뇌외상후손상경과시간제공료신적삼고지표。
Objective To observe the time-course expression of calcium-calmodulin dependent protein ki-naseⅡδ (CaMKⅡδ) in cerebral cortex after traumatic brain injury (TBI). Methods The TBI rat model was established. The expression of CaMKⅡδ in cerebral cortex around injured area was tested by Western blotting and immunohistochemical staining . Results Western blotting revealed expression of CaMKⅡδ in normal rat brain cortex. It gradually increased after TBI, peaked after 3 days, and then returned to normal level. The result of immunohistochemical staining was consistent with that of West-ern blotting. Conclusion The expression of CaMKⅡδ around injured area after TBI increased initially and then decreased. It could be used as a new indicator for wound age determination following TBI.