CAJ | 학술논문

目的：研究大鼠骨骼肌机械性损伤后不同时间点多形核白细胞（polymorphonuclear leucocytes ， PMN）、单个核细胞（mononuclear cells，MNC）及成纤维样细胞（fibroblastic cells，FBC）百分率的变化。方法建立大鼠骨骼肌机械性损伤动物模型，随机分为伤后6h、12h、1d、3d、7d、10d、14d及正常对照组。应用HE染色法和图像分析检测大鼠骨骼肌损伤后不同时间点PMN、MNC及FBC百分率。结果伤后6~12 h，损伤区内可见PMN和MNC浸润，PMN百分率达到峰值；伤后1 d，损伤区内主要以MNC浸润为主，MNC百分率达到峰值，而PMN百分率开始下降；伤后3~7 d，FBC百分率开始逐渐增加，PMN和MNC百分率则逐渐下降；伤后10~14 d，FBC百分率达到峰值。结论大鼠骨骼肌损伤区内PMN、MNC及FBC百分率呈时间规律性变化，有望成为骨骼肌损伤时间推断的参考指标。
목적：연구대서골격기궤계성손상후불동시간점다형핵백세포（polymorphonuclear leucocytes ， PMN）、단개핵세포（mononuclear cells，MNC）급성섬유양세포（fibroblastic cells，FBC）백분솔적변화。방법건립대서골격기궤계성손상동물모형，수궤분위상후6h、12h、1d、3d、7d、10d、14d급정상대조조。응용HE염색법화도상분석검측대서골격기손상후불동시간점PMN、MNC급FBC백분솔。결과상후6~12 h，손상구내가견PMN화MNC침윤，PMN백분솔체도봉치；상후1 d，손상구내주요이MNC침윤위주，MNC백분솔체도봉치，이PMN백분솔개시하강；상후3~7 d，FBC백분솔개시축점증가，PMN화MNC백분솔칙축점하강；상후10~14 d，FBC백분솔체도봉치。결론대서골격기손상구내PMN、MNC급FBC백분솔정시간규률성변화，유망성위골격기손상시간추단적삼고지표。
Objective To study the percentages of polymorphonuclear leukocytes (PMN), mononuclear cells (MNC) and fibroblastic cells (FBC) in different post-traumatic intervals after skeletal muscle me-chanical injury in rats. Methods The rat model of skeletal muscle mechanical injury was established. The rats were divided into injured groups (6 h, 12 h, 1 d, 3 d, 7 d, 10 d and 14 d after injury ) and con-trol group. The percentages of PMN, MNC and FBC in different post-traumatic intervals after skeletal muscle mechanical injury were assessed with HE staining and image analysis. Results At post-injury 6-12 h, the percentages of PMN and MNC infiltration appeared in injured sites and that of PMN reached peak. At 1 d, the percentage of MNC infiltration appeared and reached peak, while that of PMN de-creased. At 3-7 d, the percentage of FBC gradually increased, while that of PMN and MNC decreased. At 10-14 d, the percentage of FBC reached peak. Conclusion The percentages of PMN, MNC and FBC in injured zones showed time-dependent changes, which might be used as reference index for determination of age of skeletal muscle injury.