CAJ | 학술논문

目的：观察电针与塞来昔布治疗大鼠膝骨关节炎后，大鼠滑膜组织中基质金属蛋白酶-3（ MMP-3）和基质金属蛋白酶抑制剂-1（TIMP-1）含量的变化。方法：选用SD大鼠40只，采用随机数字表随机分成正常组、模型组、药物组和电针组，每组各10只。通过向SD大鼠左侧关节腔内注射单体碘乙酸钠（ MIA）并驱赶大鼠运动来建立膝骨关节炎MIA模型。造模两周后，对电针组取“后三里”穴、“阳陵泉”穴进行电针治疗；对药物组给予塞来昔布灌胃治疗；正常组跟造模组不做任何干预。结果：模型组滑膜组织中MMP-3和TIMP-1的含量较正常组显著提高（ P＜0．05），但MMP-3和TIMP-1的变化不同步；经过两周的治疗，电针组跟药物组滑膜组织中的MMP-3和TIMP-1含量较造模组显著降低（P＜0．05），但仍然略高于正常组。电针治疗组和药物治疗组中两者含量相近（P＞0．05），无统计学意义。结论：电针能有效平衡MIA膝骨关节炎模型大鼠中MMP-3和TIMP-1的异常表达，这可能是电针治疗膝骨关节炎有效的机制之一，为针灸治疗膝骨关节炎提供了有力的证据。
목적：관찰전침여새래석포치료대서슬골관절염후，대서활막조직중기질금속단백매-3（ MMP-3）화기질금속단백매억제제-1（TIMP-1）함량적변화。방법：선용SD대서40지，채용수궤수자표수궤분성정상조、모형조、약물조화전침조，매조각10지。통과향SD대서좌측관절강내주사단체전을산납（ MIA）병구간대서운동래건립슬골관절염MIA모형。조모량주후，대전침조취“후삼리”혈、“양릉천”혈진행전침치료；대약물조급여새래석포관위치료；정상조근조모조불주임하간예。결과：모형조활막조직중MMP-3화TIMP-1적함량교정상조현저제고（ P＜0．05），단MMP-3화TIMP-1적변화불동보；경과량주적치료，전침조근약물조활막조직중적MMP-3화TIMP-1함량교조모조현저강저（P＜0．05），단잉연략고우정상조。전침치료조화약물치료조중량자함량상근（P＞0．05），무통계학의의。결론：전침능유효평형MIA슬골관절염모형대서중MMP-3화TIMP-1적이상표체，저가능시전침치료슬골관절염유효적궤제지일，위침구치료슬골관절염제공료유력적증거。
Objective:To observe the changes of MMP -3 and TIMP-1 expression level in synovium of knee osteoarthritis rats after they were treated with electroacupuncture and oral celecoxib .Methods:Forty cases of male SD rats were randomly assigned into four groups ,and these were normal group , model group , electroacu-puncture group and celecoxib group .KOA model was constructed by injecting mono -iodoacetate ( MIA) to left articular cavity .Electroacupuncture group was treated with electroacupuncture at “Yang-Lingquan”( GB34 ) and “Hou-sanli”( ST36 ) with a needle afflicted to an electroacupuncture apparatus .Celecoxib group was ga-vaged with a dose of 3mg/(kg -1· 2ml -1).Model group received no treatment.And normal group was estab-lished as blank .Results:MMP-3 and TIMP-1 in synovium of model group were significantly increased ( P<0 .05 ) compared with that in the normal group in the fourth week after treatment ,and the balance between them was significantly broken;the contents of MMP -3 and TIMP -1 in electroacupuncture group and celecoxib group were reduced compared with that in the model group ( P<0.05) and they were back to be balance again after treatment.In addition,there was no great difference between two groups (P >0.05).Conclusion:This sduty demonstrates that electroacupuncture can effectively correct the imbalance between MMP -3 and TIMP-1 in synovium of knee osteoarthritis model rats .It may be one of the mechanisms in the treatment of knee osteo-arthritis by electroacupuncture ,which offers powerful support for acupuncture treatment of KOA .