风湿病与关节炎
風濕病與關節炎
풍습병여관절염
Rheumatism and Arthritis
2014年
6期
23-26
,共4页
付长龙%潘彩彬%刘国强%陈星强%林平冬%刘献祥%叶蕻芝
付長龍%潘綵彬%劉國彊%陳星彊%林平鼕%劉獻祥%葉蕻芝
부장룡%반채빈%류국강%진성강%림평동%류헌상%협홍지
软骨细胞%增殖%药对巴戟天-杜仲%多糖%含量测定%大鼠
軟骨細胞%增殖%藥對巴戟天-杜仲%多糖%含量測定%大鼠
연골세포%증식%약대파극천-두중%다당%함량측정%대서
cartilage cell%proliferation%couplet drugs of Bajitian(Radix Morindae Ofifcinalis)and Du-zhong(Cortex Eucommiae)%polysaccha-ride%content measurement%rats
目的：研究药对巴戟天-杜仲总多糖对体外培养的大鼠软骨细胞增殖活性的影响。方法：①用水提醇沉法、Sevag法除蛋白提取纯化获得其总多糖，苯酚-硫酸比色法测定其总多糖含量；②MTT法分别检测体外培养大鼠软骨细胞在800，400，200，100，50μg·mL-1总多糖DMEM溶液中24，48，72 h的增殖能力。结果：①葡萄糖标准品线性回归方程为y=0.0100x-0.0018（r=0.9996），葡萄糖标准品在10～100μg·mL-1范围内线性关系良好，平均加样回收率为99.59%，RSD为0.40%；多糖含量为43.88%。②巴戟天-杜仲总多糖对软骨细胞具有一定的促增殖作用，当作用时间为48 h、多糖浓度为400μg·mL-1时，促增殖作用最强。结论：①苯酚-硫酸比色法可作为药对巴戟天-杜仲总多糖的含量测定方法；②巴戟天-杜仲总多糖对软骨细胞的促增殖作用具有一定的时效量效关系。
目的：研究藥對巴戟天-杜仲總多糖對體外培養的大鼠軟骨細胞增殖活性的影響。方法：①用水提醇沉法、Sevag法除蛋白提取純化穫得其總多糖，苯酚-硫痠比色法測定其總多糖含量；②MTT法分彆檢測體外培養大鼠軟骨細胞在800，400，200，100，50μg·mL-1總多糖DMEM溶液中24，48，72 h的增殖能力。結果：①葡萄糖標準品線性迴歸方程為y=0.0100x-0.0018（r=0.9996），葡萄糖標準品在10～100μg·mL-1範圍內線性關繫良好，平均加樣迴收率為99.59%，RSD為0.40%；多糖含量為43.88%。②巴戟天-杜仲總多糖對軟骨細胞具有一定的促增殖作用，噹作用時間為48 h、多糖濃度為400μg·mL-1時，促增殖作用最彊。結論：①苯酚-硫痠比色法可作為藥對巴戟天-杜仲總多糖的含量測定方法；②巴戟天-杜仲總多糖對軟骨細胞的促增殖作用具有一定的時效量效關繫。
목적：연구약대파극천-두중총다당대체외배양적대서연골세포증식활성적영향。방법：①용수제순침법、Sevag법제단백제취순화획득기총다당，분분-류산비색법측정기총다당함량；②MTT법분별검측체외배양대서연골세포재800，400，200，100，50μg·mL-1총다당DMEM용액중24，48，72 h적증식능력。결과：①포도당표준품선성회귀방정위y=0.0100x-0.0018（r=0.9996），포도당표준품재10～100μg·mL-1범위내선성관계량호，평균가양회수솔위99.59%，RSD위0.40%；다당함량위43.88%。②파극천-두중총다당대연골세포구유일정적촉증식작용，당작용시간위48 h、다당농도위400μg·mL-1시，촉증식작용최강。결론：①분분-류산비색법가작위약대파극천-두중총다당적함량측정방법；②파극천-두중총다당대연골세포적촉증식작용구유일정적시효량효관계。
[ABSTRACT]Objective:To study the effects of polysaccharide in couplet drugs of Bajitian(Radix Morindae Officinalis)and Duzhong(Cortex Eucommiae)on the proliferation of rat chondrocytes cultured in vitro.Methods: ① The total polysaccharide was extracted and purified by the water extraction and alcohol pre-cipitation and the Sevag method,measuring its content by Phenol-sulfuric acid colorimetric method; ② By the MTT method,the reproductive activity of rat chondrocytes was detected in the polysaccharide DMEM solution of 800,400,200,100,50 μg·mL-1 for 24,48,72 hs.Results: ① The standard glucose linear regression equation was y=0.010 0x-0.001 8 (r=0.999 6).The linear relationship of standard glucose was good in 10~100 μg·mL-1, the average recovery being 99.59%,RSD being 0.40%and the content of polysaccharide being 43.88%. ② The total polysaccharides of Bajitian(Radix Morindae Ofifcinalis)and Duzhong(Cortex Eucommiae)could pro-mote the reproductive activity of rat chondrocytes.The promoting function was strongest when the concentration of polysaccharide was 400 μg·mL-1 for 48 hs.Conclusion:①The Phenol-sulfuric acid colorimetric method can be used to measure the content of polysaccharide in couplet drugs of Bajitian(Radix Morindae Ofifcinalis)and Duzhong(Cortex Eucommiae);②The function of polysaccharide in promoting the reproductive activity of rat chondrocytes is related to a certain time,quantity and effectiveness.
