光谱学与光谱分析
光譜學與光譜分析
광보학여광보분석
SPECTROSCOPY AND SPECTRAL ANALYSIS
2014年
7期
1917-1921
,共5页
宋晓宇%王纪华%杨贵军%崔贝%常红
宋曉宇%王紀華%楊貴軍%崔貝%常紅
송효우%왕기화%양귀군%최패%상홍
冬小麦%氮素含量%冠层氮素密度%籽粒蛋白质含量%SPAD%SFR
鼕小麥%氮素含量%冠層氮素密度%籽粒蛋白質含量%SPAD%SFR
동소맥%담소함량%관층담소밀도%자립단백질함량%SPAD%SFR
Winter wheat%Nitrogen content%Canopy nitrogen density%Grain protein content (GPC)%SPAD%SFR
小麦籽粒蛋白质含量是衡量小麦营养品质的重要指标，实现小麦品质快速的预测预报对于粮食收购部门和加工企业具有重要意义。研究基于作物叶绿素/氮素速测仪SPAD及M ultiplex 3，获取冬小麦不同生育期叶片及冠层叶绿素参数，从小麦个体及群体参量两方面进行冬小麦收获期籽粒蛋白质含量及蛋白产量的预测研究。试验于2012年4-6月在国家精准农业研究示范基地开展，研究结果表明，冬小麦返青至灌浆初期，小麦冠层氮素密度与籽粒蛋白质含量的相关性优于叶片氮素含量与蛋白质含量的相关性，灌浆中期两者与籽粒蛋白质含量相关性差别不大；小麦叶片SPAD值与叶片氮素含量相关性总体优于其与冠层氮素密度的相关性，而叶绿素荧光参数SFR_G ，SFR_R与冠层氮素密度的相关性优于其与叶片氮素含量的相关性；叶片SPAD与籽粒蛋白质含量的相关性在拔节期最弱，在灌浆中期最强，小麦冠层叶绿素荧光参数SFR_G ，SFR_R与籽粒蛋白质含量相关性在返青至拔节期不显著，但孕穗期开始显著相关，在灌浆中期相关性最强且明显优于同期叶片SPAD与籽粒蛋白质含量的相关性；冬小麦籽粒蛋白产量与叶片SPAD值在小麦孕穗期至灌浆期显著相关，与SFR_G和SFR_R在小麦灌浆期显著相关；研究基于灌浆中期SPAD值及SFR_R值，构建了冬小麦籽粒蛋白质含量及籽粒蛋白产量的预测模型，其中，籽粒蛋白质含量预测模型复相关指数分别为0.426和0.497，模型标准误差分别为0.060％和0.055％，籽粒蛋白产量预测模型复相关指数分别为0.366和0.386，模型标准误差分别为125.367和123.454 kg · ha-1。研究表明，利用叶片SPAD值及冠层叶绿素荧光信息，在小麦收获前进行品质的快速预测是可行的。
小麥籽粒蛋白質含量是衡量小麥營養品質的重要指標，實現小麥品質快速的預測預報對于糧食收購部門和加工企業具有重要意義。研究基于作物葉綠素/氮素速測儀SPAD及M ultiplex 3，穫取鼕小麥不同生育期葉片及冠層葉綠素參數，從小麥箇體及群體參量兩方麵進行鼕小麥收穫期籽粒蛋白質含量及蛋白產量的預測研究。試驗于2012年4-6月在國傢精準農業研究示範基地開展，研究結果錶明，鼕小麥返青至灌漿初期，小麥冠層氮素密度與籽粒蛋白質含量的相關性優于葉片氮素含量與蛋白質含量的相關性，灌漿中期兩者與籽粒蛋白質含量相關性差彆不大；小麥葉片SPAD值與葉片氮素含量相關性總體優于其與冠層氮素密度的相關性，而葉綠素熒光參數SFR_G ，SFR_R與冠層氮素密度的相關性優于其與葉片氮素含量的相關性；葉片SPAD與籽粒蛋白質含量的相關性在拔節期最弱，在灌漿中期最彊，小麥冠層葉綠素熒光參數SFR_G ，SFR_R與籽粒蛋白質含量相關性在返青至拔節期不顯著，但孕穗期開始顯著相關，在灌漿中期相關性最彊且明顯優于同期葉片SPAD與籽粒蛋白質含量的相關性；鼕小麥籽粒蛋白產量與葉片SPAD值在小麥孕穗期至灌漿期顯著相關，與SFR_G和SFR_R在小麥灌漿期顯著相關；研究基于灌漿中期SPAD值及SFR_R值，構建瞭鼕小麥籽粒蛋白質含量及籽粒蛋白產量的預測模型，其中，籽粒蛋白質含量預測模型複相關指數分彆為0.426和0.497，模型標準誤差分彆為0.060％和0.055％，籽粒蛋白產量預測模型複相關指數分彆為0.366和0.386，模型標準誤差分彆為125.367和123.454 kg · ha-1。研究錶明，利用葉片SPAD值及冠層葉綠素熒光信息，在小麥收穫前進行品質的快速預測是可行的。
소맥자립단백질함량시형량소맥영양품질적중요지표，실현소맥품질쾌속적예측예보대우양식수구부문화가공기업구유중요의의。연구기우작물협록소/담소속측의SPAD급M ultiplex 3，획취동소맥불동생육기협편급관층협록소삼수，종소맥개체급군체삼량량방면진행동소맥수획기자립단백질함량급단백산량적예측연구。시험우2012년4-6월재국가정준농업연구시범기지개전，연구결과표명，동소맥반청지관장초기，소맥관층담소밀도여자립단백질함량적상관성우우협편담소함량여단백질함량적상관성，관장중기량자여자립단백질함량상관성차별불대；소맥협편SPAD치여협편담소함량상관성총체우우기여관층담소밀도적상관성，이협록소형광삼수SFR_G ，SFR_R여관층담소밀도적상관성우우기여협편담소함량적상관성；협편SPAD여자립단백질함량적상관성재발절기최약，재관장중기최강，소맥관층협록소형광삼수SFR_G ，SFR_R여자립단백질함량상관성재반청지발절기불현저，단잉수기개시현저상관，재관장중기상관성최강차명현우우동기협편SPAD여자립단백질함량적상관성；동소맥자립단백산량여협편SPAD치재소맥잉수기지관장기현저상관，여SFR_G화SFR_R재소맥관장기현저상관；연구기우관장중기SPAD치급SFR_R치，구건료동소맥자립단백질함량급자립단백산량적예측모형，기중，자립단백질함량예측모형복상관지수분별위0.426화0.497，모형표준오차분별위0.060％화0.055％，자립단백산량예측모형복상관지수분별위0.366화0.386，모형표준오차분별위125.367화123.454 kg · ha-1。연구표명，이용협편SPAD치급관층협록소형광신식，재소맥수획전진행품질적쾌속예측시가행적。
The present study focused on the wheat harvest grain protein content (GPC) estimation based on wheat leaf and cano-py chlorophyll parameters ,SPAD and SFR ,which were acquired by two hand-held instruments ,SPAD and Multiplex 3 .The wheat GPC estimate experiment was applied on a wheat field of the Scientific Observation and Experiment Field Station for Preci-sion Agriculture at suburb of Beijing in 2012 .The wheat leaf SPAD and canopy SFR value were measured in field for all 110 wheat sample points at five different wheat growth stages from April to June .The wheat plant sample for each point was then collected after the SPAD and SFR measurement and sent to lab for leaf nitrogen content (LNC ) and canopy nitrogen density (CND) analysis .Analysis results showed that the correlation coefficients of wheat GPC with wheat CND were much higher than that from wheat tillering stage to early milking stage .They were similar at the wheat middle milking stage .While the wheat leaf SPAD value was highly correlated with wheat LNC at wheat tillering ,heading and early milking stage .Wheat canopy chloro-phyll parameters SFR were highly correlated with wheat CND at wheat tillering ,jointing ,heading and milking stage .It can be seen from the study that SFR is more sensitive to the wheat CND compared with wheat LNC .The analysis also indicated that leaf SPAD value at wheat tillering ,heading and milking stage was highly correlated with wheat GPC and yield of grain protein (YGP) .The wheat canopy parameters ,SFR_G and SFR_R were significantly correlated with wheat GPC and YGP at wheat milking stage .Then the optimal GPC and YGP estimation model was established .The R2 of GPC estimation models established by SPAD and SFR_R are 0.426 and 0.497 ,and the standard errors of the estimate are 0.060% and 0.055% ,respectively .The R2 of YGP estimation models established by SPAD and SFR_R are 0.366 and 0.386 and the standard errors of the estimate are 125.367 and 123.454 kg · ha-1 ,respectively .The study reveals that SPAD value is a good indicator of single plant activity while SFR_G and SFR_R are better indicators for the wheat group activity .Wheat leaf SPAD value and canopy chlorophyll fluo-rescence information SFR are all feasible and valuable for GPC estimation before wheat harvesting .
