CAJ | 학술논문

目的：茜草对骨骼具有红染的效果，探索茜草如何影响骨骼的显微结构，是否饲喂茜草对大鼠的内脏器官具有显著的毒害作用。方法本试验选取80只1月龄大鼠，随机分为4组（分别添加茜草量为0％、5％、10％和15％入日粮中），饲喂后0.5、1、3和6个月，采取双侧股骨、内脏和血液。结果饲喂茜草6个月后，10％和15％组大鼠股骨骨干的平均厚度、骨干内径、骨干外径、骨髓腔面积、皮质骨面积、骨干总面积和股骨骨干的骨矿物质密度显著高于0％组大鼠；股骨远端骨小梁体积百分比，骨小梁厚度，连接密度，骨小梁数量和股骨远端的骨矿物质密度的水平均显著高于0％组大鼠，而股骨远端的骨小梁分离度和结构模型指数显著低于0％组大鼠。但是饲喂茜草6个月后，15％组的大鼠血液中尿素氮和肌酐的含量与0％组大鼠之间差异显著。结论饲喂10％茜草对大鼠无毒性影响，并能促进大鼠的骨骼长度和骨的显微结构，为以后茜草的合理应用提供了实验基础。
목적：천초대골격구유홍염적효과，탐색천초여하영향골격적현미결구，시부사위천초대대서적내장기관구유현저적독해작용。방법본시험선취80지1월령대서，수궤분위4조（분별첨가천초량위0％、5％、10％화15％입일량중），사위후0.5、1、3화6개월，채취쌍측고골、내장화혈액。결과사위천초6개월후，10％화15％조대서고골골간적평균후도、골간내경、골간외경、골수강면적、피질골면적、골간총면적화고골골간적골광물질밀도현저고우0％조대서；고골원단골소량체적백분비，골소량후도，련접밀도，골소량수량화고골원단적골광물질밀도적수평균현저고우0％조대서，이고골원단적골소량분리도화결구모형지수현저저우0％조대서。단시사위천초6개월후，15％조적대서혈액중뇨소담화기항적함량여0％조대서지간차이현저。결론사위10％천초대대서무독성영향，병능촉진대서적골격장도화골적현미결구，위이후천초적합리응용제공료실험기출。
Objective Bone could be stained with red color by madder.However, whether feeding with the madder has significantly toxicity in animals was still unknown.This study was designed to explore this question.Methods Eighty 1-month rats were selected in this study, and then randomly divided into 6 groups (0%, 5%, 10%, or 15%madder added in diet).After 0.5-, 1-, 3-, and 6-month feeding, all the rats were executed.And the bilateral femur, the internal organs, and the blood were collected.Results The results showed that the mean thickness, the inner diameter, the external diameter, the marrow cavity area, the cortical bone area, the total area, and the bone mineral density of the femur diaphysis in 10% group and 15%group increased significantly compared with those in 0%group.The trabecular bone volume, the trabecular thickness, the connectivity density, the trabecular number, and the bone mineral density of the distal femur in 10% group were higher than those in 0% group.The trabecular separation and the structure model index of the distal femur in 10%group were lower than those in 0%group.However, after 6-month feeding, the blood levels of urea nitrogen and creatinine in 15% group were significantly different with that in 0%group.Conclusion There is no toxicity of 10%madder in the diet for rats.Madder can improve the length of the femur and the bone micro-structure, which provides the experimental basis for the reasonable application of madder.