中华耳科学杂志
中華耳科學雜誌
중화이과학잡지
CHINESE JOURNAL OF OTOLOGY
2014年
2期
328-330
,共3页
自身免疫%耳蜗%豚鼠%听功能
自身免疫%耳蝸%豚鼠%聽功能
자신면역%이와%돈서%은공능
Autoimmunity%Cochlea%Guinea pig%Hearing function
目的：探讨环磷酰胺在制备豚鼠自身免疫性耳聋动物模型中的作用。方法白色红目豚鼠30只,配对设计分成3组各10只。实验组豚鼠腹腔注射环磷酰胺(150mg/kg )进行预处理，2 d后,每只豚鼠将粗制内耳抗原（crude in-ner ear antigens，CIEAgs）400μg ·（0.2ml）-1加等量完全弗氏佐剂进行后足垫、背部皮内多点注射免疫；对照1组用CIEAgs 400μg·（0.2ml）-1加等量完全弗氏佐剂免疫动物；对照2组仅行环磷酰胺预处理。所有动物免疫前、免疫后7、14、21d ,接受听性脑干诱发电位(auditory brain-stem response，ABR)检测。结果免疫前所有豚鼠ABR反应阈均值为29.50±5.56dBSPL，免疫后7天实验组、对照1及对照2组ABR反应阈分别为52.50±12.72、36.50±11.25、31.50±3.37；免疫后14天时三组反应域分别为58.50±14.79、33.25±8.93、30.50±3.69 dBSPL；免疫后21天分别为46.25±12.45、30.00±4.29、30.00±3.33 dBSPL。免疫后,实验组动物各时间段反应阈高于对照1,2组( P<0.01)。免疫前后组内比较，实验组免疫后7天、14天、21天反应域高于实验前（P<0.01)；对照1组仅免疫后7天高于免疫前(P<0.05),14天、21天与免疫前相比差异无显著性，对照2组各时间段与免疫前相比差异均无显著性。结论将豚鼠用环磷酰胺进行预处理，可以显著提高粗制内耳抗原建立自身免疫性耳聋动物模型的成功率，并显著提高其ABR阈值，而环磷酰胺本身对豚鼠内耳听力没有显著影响。
目的：探討環燐酰胺在製備豚鼠自身免疫性耳聾動物模型中的作用。方法白色紅目豚鼠30隻,配對設計分成3組各10隻。實驗組豚鼠腹腔註射環燐酰胺(150mg/kg )進行預處理，2 d後,每隻豚鼠將粗製內耳抗原（crude in-ner ear antigens，CIEAgs）400μg ·（0.2ml）-1加等量完全弗氏佐劑進行後足墊、揹部皮內多點註射免疫；對照1組用CIEAgs 400μg·（0.2ml）-1加等量完全弗氏佐劑免疫動物；對照2組僅行環燐酰胺預處理。所有動物免疫前、免疫後7、14、21d ,接受聽性腦榦誘髮電位(auditory brain-stem response，ABR)檢測。結果免疫前所有豚鼠ABR反應閾均值為29.50±5.56dBSPL，免疫後7天實驗組、對照1及對照2組ABR反應閾分彆為52.50±12.72、36.50±11.25、31.50±3.37；免疫後14天時三組反應域分彆為58.50±14.79、33.25±8.93、30.50±3.69 dBSPL；免疫後21天分彆為46.25±12.45、30.00±4.29、30.00±3.33 dBSPL。免疫後,實驗組動物各時間段反應閾高于對照1,2組( P<0.01)。免疫前後組內比較，實驗組免疫後7天、14天、21天反應域高于實驗前（P<0.01)；對照1組僅免疫後7天高于免疫前(P<0.05),14天、21天與免疫前相比差異無顯著性，對照2組各時間段與免疫前相比差異均無顯著性。結論將豚鼠用環燐酰胺進行預處理，可以顯著提高粗製內耳抗原建立自身免疫性耳聾動物模型的成功率，併顯著提高其ABR閾值，而環燐酰胺本身對豚鼠內耳聽力沒有顯著影響。
목적：탐토배린선알재제비돈서자신면역성이롱동물모형중적작용。방법백색홍목돈서30지,배대설계분성3조각10지。실험조돈서복강주사배린선알(150mg/kg )진행예처리，2 d후,매지돈서장조제내이항원（crude in-ner ear antigens，CIEAgs）400μg ·（0.2ml）-1가등량완전불씨좌제진행후족점、배부피내다점주사면역；대조1조용CIEAgs 400μg·（0.2ml）-1가등량완전불씨좌제면역동물；대조2조부행배린선알예처리。소유동물면역전、면역후7、14、21d ,접수은성뇌간유발전위(auditory brain-stem response，ABR)검측。결과면역전소유돈서ABR반응역균치위29.50±5.56dBSPL，면역후7천실험조、대조1급대조2조ABR반응역분별위52.50±12.72、36.50±11.25、31.50±3.37；면역후14천시삼조반응역분별위58.50±14.79、33.25±8.93、30.50±3.69 dBSPL；면역후21천분별위46.25±12.45、30.00±4.29、30.00±3.33 dBSPL。면역후,실험조동물각시간단반응역고우대조1,2조( P<0.01)。면역전후조내비교，실험조면역후7천、14천、21천반응역고우실험전（P<0.01)；대조1조부면역후7천고우면역전(P<0.05),14천、21천여면역전상비차이무현저성，대조2조각시간단여면역전상비차이균무현저성。결론장돈서용배린선알진행예처리，가이현저제고조제내이항원건립자신면역성이롱동물모형적성공솔，병현저제고기ABR역치，이배린선알본신대돈서내이은력몰유현저영향。
Objective To study the effects of cyclophosphamide in guinea pigs with autoimmune deafness. Methods Guinea pigs were randomly divided into three groups (10 in each). One group of animals received intracutaneous inoculation of an emulsion consisting of equal parts of crude inner ear antigens (CIEAgs, 0.2 ml) and complete Freund’s adjuvant (CFA) at multiple sites two days after pretreatment with cyclophosphamide (Experimental Group). The second group of animals received only inoculation of CIEAgs and CFA (Control Group #1), and the rest animals received only cyclophosphamide pretreatment (Control Group #2). Animals in all three groups received auditory brain-stem response (ABR) testing before and on Days 7, 14 and 21 after immunization. Results The mean ABR threshold across all animals was 29.50±5.56 dB SPL before immunization. After immunization, the threshold was 52.50±12.72 dB SPL, 36.50±11.25 dB SPL and 31.50±3.37 dB SPL on Day 7, 58.50± 14.79 dB SPL, 33.25 ± 8.93 dB SPL and 30.50 ± 3.69 dB SPL on Day 14, and 46.25 ± 12.45 dB SPL, 30.00 ± 4.29 dB SPL and 30.00±3.33 dB SPL on Day 21 or the experimental group, Control Group 1 and Control Group 2, respectively. The ABR thresh-old for the experimental group on all after immunization days was higher than those for the two control groups (P<0.01) and than before immunization (P<0.01). The ABR threshold for Control Group 1 was higher than before immunization on post-im-munization Day 7 only (P<0.05), and the ABR threshold for Control Group 2 showed no significant post-immunization change. Conclusions After pretreatment with cyclophosphamide, single inoculation of CIEAgs-CFA emulsion can significantly in-crease ABR thresholds in guinea pigs.
