中华脑科疾病与康复杂志(电子版)
中華腦科疾病與康複雜誌(電子版)
중화뇌과질병여강복잡지(전자판)
CHINESE JOURNAL OF BRAIN DI8SEASES AND REHABILITATIN(ELECTRONIC EDITION)
2014年
2期
31-34
,共4页
朱岩岩%李彤%李合华%宋志秀%张平%闫海清%常利
硃巖巖%李彤%李閤華%宋誌秀%張平%閆海清%常利
주암암%리동%리합화%송지수%장평%염해청%상리
高血压%高脂血症%肾动脉梗阻%动脉粥样硬化
高血壓%高脂血癥%腎動脈梗阻%動脈粥樣硬化
고혈압%고지혈증%신동맥경조%동맥죽양경화
Hypertension%Hyperlipidemias%Renal artery obstruction%Atherosclerosis
目的:通过高血压合并高血脂建立脑动脉粥样硬化( CAS )大鼠模型。方法20只成年雌性Sprague-Dawley大鼠随机分成模型组(10只)和正常组(10只),模型组通过双侧肾动脉狭窄术造成实验性高血压,术后喂养高脂饲料16周,正常组只行肾动脉分离术,术后喂养普通饲料16周。实验前后测定血压、血脂水平,同时采用苏木素-伊红染色( HE染色法)观察颈内动脉血管形态学改变。组间检测数据比较采用配对样本t检验。结果模型组大鼠实验后与实验前相比,血压、总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)均显著性升高(t血压=10.665,tTC =26.357, tTG =11.121,tLDL-C =15.961,P均<0.05),高密度脂蛋白胆固醇(HDL-C)变化无统计学差异(tHDL-C =0.173,P>0.05),颈内动脉终段出现动脉粥样硬化斑块;正常组大鼠实验后与实验前相比,血压、TC、TG、LDL-C、HDL-C均无统计学差异(t血压=1.182,tTC =0.654,tTG =1.202,tLDL-C=0.712,tHDL-C =1.462, P均>0.05),血管无明显病变。结论通过高血压合并高血脂可成功建立病理改变与人相似、稳定的适合于缺血性脑血管病研究的大鼠CAS模型。
目的:通過高血壓閤併高血脂建立腦動脈粥樣硬化( CAS )大鼠模型。方法20隻成年雌性Sprague-Dawley大鼠隨機分成模型組(10隻)和正常組(10隻),模型組通過雙側腎動脈狹窄術造成實驗性高血壓,術後餵養高脂飼料16週,正常組隻行腎動脈分離術,術後餵養普通飼料16週。實驗前後測定血壓、血脂水平,同時採用囌木素-伊紅染色( HE染色法)觀察頸內動脈血管形態學改變。組間檢測數據比較採用配對樣本t檢驗。結果模型組大鼠實驗後與實驗前相比,血壓、總膽固醇(TC)、甘油三酯(TG)和低密度脂蛋白膽固醇(LDL-C)均顯著性升高(t血壓=10.665,tTC =26.357, tTG =11.121,tLDL-C =15.961,P均<0.05),高密度脂蛋白膽固醇(HDL-C)變化無統計學差異(tHDL-C =0.173,P>0.05),頸內動脈終段齣現動脈粥樣硬化斑塊;正常組大鼠實驗後與實驗前相比,血壓、TC、TG、LDL-C、HDL-C均無統計學差異(t血壓=1.182,tTC =0.654,tTG =1.202,tLDL-C=0.712,tHDL-C =1.462, P均>0.05),血管無明顯病變。結論通過高血壓閤併高血脂可成功建立病理改變與人相似、穩定的適閤于缺血性腦血管病研究的大鼠CAS模型。
목적:통과고혈압합병고혈지건립뇌동맥죽양경화( CAS )대서모형。방법20지성년자성Sprague-Dawley대서수궤분성모형조(10지)화정상조(10지),모형조통과쌍측신동맥협착술조성실험성고혈압,술후위양고지사료16주,정상조지행신동맥분리술,술후위양보통사료16주。실험전후측정혈압、혈지수평,동시채용소목소-이홍염색( HE염색법)관찰경내동맥혈관형태학개변。조간검측수거비교채용배대양본t검험。결과모형조대서실험후여실험전상비,혈압、총담고순(TC)、감유삼지(TG)화저밀도지단백담고순(LDL-C)균현저성승고(t혈압=10.665,tTC =26.357, tTG =11.121,tLDL-C =15.961,P균<0.05),고밀도지단백담고순(HDL-C)변화무통계학차이(tHDL-C =0.173,P>0.05),경내동맥종단출현동맥죽양경화반괴;정상조대서실험후여실험전상비,혈압、TC、TG、LDL-C、HDL-C균무통계학차이(t혈압=1.182,tTC =0.654,tTG =1.202,tLDL-C=0.712,tHDL-C =1.462, P균>0.05),혈관무명현병변。결론통과고혈압합병고혈지가성공건립병리개변여인상사、은정적괄합우결혈성뇌혈관병연구적대서CAS모형。
Objective Cerebral atherosclerosis ( CAS ) rat model established by hypertension and high cholesterol .Methods Twenty adult female Sprague-Dawley rats were equally randomized into model group(10 rats)and normal group(10 rats).Model group was made by bilateral renal artery stenosis and fed high fat diet for 16 weeks.While control group was made by renal artery dissection and fed basic food for 16 weeks.Blood pressure and blood lipid levels were measured before and after the experiment , while morphological changes of the internal carotid artery was observed by Hematoxylin-eosin( HE) staining.Data were analyzed by paired samples t test.Results Compared with the previous experiments ,the blood pressure (BP),total cholesterol(TC),total triglyceride(TG)and low-density lipoprotein-cholesterol(LDL-C)level of model group rats after operation had significantly higher than that before operation (tBP =10.665,tTC =26.357,tTG =11.121,tLDL-C =15.961,all P <0.05),high-density lipoprotein-cholesterol(HDL-C)had no significant difference(tHDL-C =0.173,P>0.05),The terminal segment of the internal carotid artery appear atherosclerotic plaques .Compared the previous experiments , the BP, TC, TG, LDL-C and HLD-C level of normal group rats after operation had no significant difference (tBP =1.182,tTC =0.654,tTG =1.202,tLDL-C =0.712,tHDL-C =1.462,all P>0.05),no vascular lesions.Conclusion CAS rat models are established by hypertension and high cholesterol ,the pathological changes similar to human ,stable and suitable for ischemic cerebrovascular disease research .