CAJ | 학술논문

目的：观察胆红素对离体培养的新生SD大鼠耳蜗器官的损伤效应。方法健康新生3日龄SD大鼠18只，雌雄不限，分离其双侧的内耳共得到耳蜗器官组织36个，体外培养，12h后换液。选择贴壁、存活良好的组织30个入选实验，并随机分为两组(15个/组)：实验组于贴壁第二天开始进行胆红素处理,对照组加入相应体积的生理盐水。处理后24h，实验组和对照组同时终止培养，并对其进行固定、免疫组织化学染色,共聚焦显微镜观察毛细胞及螺旋神经节神经元的形态变化并计数，统计对比。结果胆红素组离体培养的耳蜗器官中螺旋神经节神经元胞体及其树突纤维与对照组相比有明显的减少，内外毛细胞形态完整，数量无明显变化。结论大鼠耳蜗器官螺旋神经节神经元对胆红素的毒性敏感，而内外毛细胞对其毒性相对不敏感。
목적：관찰담홍소대리체배양적신생SD대서이와기관적손상효응。방법건강신생3일령SD대서18지，자웅불한，분리기쌍측적내이공득도이와기관조직36개，체외배양，12h후환액。선택첩벽、존활량호적조직30개입선실험，병수궤분위량조(15개/조)：실험조우첩벽제이천개시진행담홍소처리,대조조가입상응체적적생리염수。처리후24h，실험조화대조조동시종지배양，병대기진행고정、면역조직화학염색,공취초현미경관찰모세포급라선신경절신경원적형태변화병계수，통계대비。결과담홍소조리체배양적이와기관중라선신경절신경원포체급기수돌섬유여대조조상비유명현적감소，내외모세포형태완정，수량무명현변화。결론대서이와기관라선신경절신경원대담홍소적독성민감，이내외모세포대기독성상대불민감。
Objective To investigate the toxic effects of bilirubin on the cultured P3 rat cochlear in vitro. Methods Thir-ty-six cochlear basilar membranes including spiral ganglion neuron were gently isolated from eighteen healthy P3 Sprague-Dawley (SD) rats of both genders. After 12 hour's primary culturing, the culture medium was removed and replaced with 5%FBS fresh medium. 6 samples were not in good survival shape and were discarded.The other 30 samples in good sur-vival shape were randomly divided into two groups (15 each) and treated with normal saline (control group) or bilirubin (experi-mental group) respectively. After 24h’s culture, all the samples in the both groups were fixed and underwent immunofluores-cence staining. The morphology of the SGNs or HCs were observed under laser confocal microscope. The numbers of the nerve fibers and hair cells were counted and underwent statistical analysis. Results The number of the nerve fibers in the experi-mental group were reduced significantly compared with that of the control group. The shape and number of the hair cells (inner or outer) didn't change significantly. Conclusion The spiral ganglion neurons are sensitive to bilirubin in vitro but the inner and outer hair cells are not sensitive to bilirubin.