CAJ | 학술논문

为了解赣榆县水产养殖环境抗生素耐药基因的传播机制，对从该环境筛选得到的多重耐药细菌进行整合子-基因盒检测，并对底泥环境中微生物群落多样性进行调查。实验发现，在66株被检测的细菌中有42株携带Ⅰ型整合子，Ⅱ型整合子全部呈阴性。基因盒可变区片断PCR扩增，测序结果表明，甲氧苄啶类、氨基糖甙类耐药基因的形成与基因盒捕获有关。整合子-基因盒系统在多重耐药基因传播中起重要作用。16 S rRNA克隆文库分析表明，养殖区底泥微生物群落多样性丰富，为抗性基因的传播提供了广泛宿主。
위료해공유현수산양식배경항생소내약기인적전파궤제，대종해배경사선득도적다중내약세균진행정합자-기인합검측，병대저니배경중미생물군락다양성진행조사。실험발현，재66주피검측적세균중유42주휴대Ⅰ형정합자，Ⅱ형정합자전부정음성。기인합가변구편단PCR확증，측서결과표명，갑양변정류、안기당대류내약기인적형성여기인합포획유관。정합자-기인합계통재다중내약기인전파중기중요작용。16 S rRNA극륭문고분석표명，양식구저니미생물군락다양성봉부，위항성기인적전파제공료엄범숙주。
To investigate the dissemination mechanism of resistance gene in aquaculture environ-ments of Ganyu County,integron-cassettes were determined in 6 6 multidrug resistance isolates and the microbial community diversity of sediments was investigated.Class Ⅰ integrons were present in 42 isolates and classⅡ integrons were negative.By PCR of variable region,the resist-ance to trimethoprim and aminoglycosides were strongly associated with cassettes.The results show that integrons play a maj or role in the spread of multidrug resistance.The diversity of mi-crobial community is rich according to the 16S rRNA gene library,which can provide a broad host for the dissemination of resistance genes.