中医药学报
中醫藥學報
중의약학보
ACTA CHINESE MEDICINE AND PHARMACOLOGY
2014年
3期
18-22
,共5页
沈小雨%郭孟玮%杨佳敏%赵雅芳%许咏思%罗丽%齐丹丹%张玲%申松希%李晓泓%朱江%任晓暄%张露芬
瀋小雨%郭孟瑋%楊佳敏%趙雅芳%許詠思%囉麗%齊丹丹%張玲%申鬆希%李曉泓%硃江%任曉暄%張露芬
침소우%곽맹위%양가민%조아방%허영사%라려%제단단%장령%신송희%리효홍%주강%임효훤%장로분
寒凝痛经%子宫微循环%刺激量
寒凝痛經%子宮微循環%刺激量
한응통경%자궁미순배%자격량
Dysmenorrhea with cold syndrome%Uterine microcirculation%Quantity of acupuncture stimulus
目的：观察不同针刺刺激量对寒凝证类痛经大鼠各时段子宫微循环的影响，探讨刺激量与针刺效应之间的关系。方法：将动情间期的SD（ Spraguc Dawlcy ）雌性大鼠随机分为4组，分别是盐水组、模型组、刺激量A组（期望“得气”组）、刺激量B组（期望“不得气”组），每组8只。模型组和针刺组采用全身冷冻（-25℃环境下冷冻4 h/天/5天）配合激素注射方法（每日给予大鼠皮下注射苯甲酸雌二醇注射液，连续10天，末次给药1h后，腹腔注射缩宫素2U/只），制备寒凝证类痛经大鼠模型。盐水组连续10日皮下注射等剂量的生理盐水，末次给药1h后，腹腔注射生理盐水2U/只。针刺组于第10日以不同刺激量针刺双侧“三阴交”穴20 min。采用XW-B-3冷光源微循环显微检查仪观察各组大鼠子宫微循环在不同时段的变化。结果：与盐水组比较，模型组各时段的微血管管径、毛细血管管径（除40 min、60 min外）明显收缩（ P＜0.01）、流态减慢或停滞（ P＜0．01或P＜0.05）；与模型组比较，刺激量A组各时段的微血管管径、毛细血管管径（仅5min、10min、20min、30min）明显扩张（P＜0．01或P＜0．05），各时段流态明显改善（ P＜0．01或P ＜0．05）。刺激量B 组微血管管径在30min 出现明显扩张（ P ＜0.01），微血管清晰度（仅50min、60min）有所改善（P＜0．05）；与刺激量B组比较，刺激量A组各时段的微血管管径，毛细血管管径（仅5min、10min、20min、30min）明显扩张（P＜0．01或P＜0．05），流态（除50min、60min外）明显改善（P＜0．01或P＜0．05）。结论：针刺可以改善子宫微循环障碍，不同针刺刺激量对子宫微循环的作用存在差异，刺激量A组针刺疗效显著优于刺激量B组，处于较强得气状态，间接说明了得气对针效的重要性。
目的：觀察不同針刺刺激量對寒凝證類痛經大鼠各時段子宮微循環的影響，探討刺激量與針刺效應之間的關繫。方法：將動情間期的SD（ Spraguc Dawlcy ）雌性大鼠隨機分為4組，分彆是鹽水組、模型組、刺激量A組（期望“得氣”組）、刺激量B組（期望“不得氣”組），每組8隻。模型組和針刺組採用全身冷凍（-25℃環境下冷凍4 h/天/5天）配閤激素註射方法（每日給予大鼠皮下註射苯甲痠雌二醇註射液，連續10天，末次給藥1h後，腹腔註射縮宮素2U/隻），製備寒凝證類痛經大鼠模型。鹽水組連續10日皮下註射等劑量的生理鹽水，末次給藥1h後，腹腔註射生理鹽水2U/隻。針刺組于第10日以不同刺激量針刺雙側“三陰交”穴20 min。採用XW-B-3冷光源微循環顯微檢查儀觀察各組大鼠子宮微循環在不同時段的變化。結果：與鹽水組比較，模型組各時段的微血管管徑、毛細血管管徑（除40 min、60 min外）明顯收縮（ P＜0.01）、流態減慢或停滯（ P＜0．01或P＜0.05）；與模型組比較，刺激量A組各時段的微血管管徑、毛細血管管徑（僅5min、10min、20min、30min）明顯擴張（P＜0．01或P＜0．05），各時段流態明顯改善（ P＜0．01或P ＜0．05）。刺激量B 組微血管管徑在30min 齣現明顯擴張（ P ＜0.01），微血管清晰度（僅50min、60min）有所改善（P＜0．05）；與刺激量B組比較，刺激量A組各時段的微血管管徑，毛細血管管徑（僅5min、10min、20min、30min）明顯擴張（P＜0．01或P＜0．05），流態（除50min、60min外）明顯改善（P＜0．01或P＜0．05）。結論：針刺可以改善子宮微循環障礙，不同針刺刺激量對子宮微循環的作用存在差異，刺激量A組針刺療效顯著優于刺激量B組，處于較彊得氣狀態，間接說明瞭得氣對針效的重要性。
목적：관찰불동침자자격량대한응증류통경대서각시단자궁미순배적영향，탐토자격량여침자효응지간적관계。방법：장동정간기적SD（ Spraguc Dawlcy ）자성대서수궤분위4조，분별시염수조、모형조、자격량A조（기망“득기”조）、자격량B조（기망“불득기”조），매조8지。모형조화침자조채용전신냉동（-25℃배경하냉동4 h/천/5천）배합격소주사방법（매일급여대서피하주사분갑산자이순주사액，련속10천，말차급약1h후，복강주사축궁소2U/지），제비한응증류통경대서모형。염수조련속10일피하주사등제량적생리염수，말차급약1h후，복강주사생리염수2U/지。침자조우제10일이불동자격량침자쌍측“삼음교”혈20 min。채용XW-B-3랭광원미순배현미검사의관찰각조대서자궁미순배재불동시단적변화。결과：여염수조비교，모형조각시단적미혈관관경、모세혈관관경（제40 min、60 min외）명현수축（ P＜0.01）、류태감만혹정체（ P＜0．01혹P＜0.05）；여모형조비교，자격량A조각시단적미혈관관경、모세혈관관경（부5min、10min、20min、30min）명현확장（P＜0．01혹P＜0．05），각시단류태명현개선（ P＜0．01혹P ＜0．05）。자격량B 조미혈관관경재30min 출현명현확장（ P ＜0.01），미혈관청석도（부50min、60min）유소개선（P＜0．05）；여자격량B조비교，자격량A조각시단적미혈관관경，모세혈관관경（부5min、10min、20min、30min）명현확장（P＜0．01혹P＜0．05），류태（제50min、60min외）명현개선（P＜0．01혹P＜0．05）。결론：침자가이개선자궁미순배장애，불동침자자격량대자궁미순배적작용존재차이，자격량A조침자료효현저우우자격량B조，처우교강득기상태，간접설명료득기대침효적중요성。
Objective:To observe the effect of different quantity of acupuncture stimulus on uterine microcirculation in dysmenorrhea rats with cold syndrome at different time -points,and to discuss the relationship between quantity of acu-puncture stimulus and acupuncture effect .Methods:Dioestrum female rats were randomly divided into four groups , sa-line control group, model control group, A stimuli group(“deqi”group), B stimuli group(no“deqi”group), with eight rats in each group .Rats of model control group and stimulus groups were frozen in -25℃freezer for five days with four hours per day while given subcutaneous estradiol benzoate injection for ten days with one hour after the last injection , in-tra-peritoneal injection of oxytocin 2u/each.Saline control group was given the same dose of saline for ten days with one hour after the last injection , intra-peritoneal injection of saline 2u/each.On the tenth day, stimulus groups were given different quantity of acupuncture stimulus on both sides of “Sanyinjiao(SP6)” for 20 minutes.Use XW-B-3 Cold Light microcirculation tester to observe the changes of uterine microcirculation in each group at different time -points.Results:Model control group was compared with to saline control group , and diameters of micro -vessel at all time-points, diameters of capillary (except 40min and 60min) were significantly contracted (P<0.01),blood flow slo-wer or stagnation (P<0.01 or P<0.05).Stimulus groups were compared with model control group , and about A stim-uli group, diameters of micro-vessel at all time -points, blood flow at all time -points was speeded up significantly (P<0.01).About B stimuli group, diameters of micro -vessel were expanded only at 30min-point(P<0.01).A stimuli group was compared with B stimuli group :diameters of micro -vessel at all time -points, diameters of capillary (only 5min,10min,20min and 30min)were significantly expanded (P<0.01 or P<0.05),blood flow(except 50min and 60min)were obviously improved(P<0.01 or P<0.05).Conclusion:Acupuncture could improve uterine microcir-culation.With different quantity of acupuncture stimulus , effects of uterine microcirculation were different between A stimuli group and B stimuli group ,the former was in a better and stronger“deqi”state,which showed the importance of“deqi”in acupuncture efficacy .
