中医药学报
中醫藥學報
중의약학보
ACTA CHINESE MEDICINE AND PHARMACOLOGY
2014年
3期
14-18
,共5页
胡柚%3 T3-L1细胞%葡萄糖摄取%白细胞介素-6
鬍柚%3 T3-L1細胞%葡萄糖攝取%白細胞介素-6
호유%3 T3-L1세포%포도당섭취%백세포개소-6
Citrus changshan -huyou Y.B.Chang%3T3-L1 adipocytes%Glucose uptake%IL-6
目的：用不同极性的溶剂对胡柚果肉进行依次分离，得到5个不同极性组分，将不同极性组分进行促进3 T3-L1脂肪细胞葡萄糖消耗、降低白细胞介素-6和游离脂肪酸分泌的活性组分的筛选及其机制的研究。方法：采用溶剂法将胡柚果肉分离成5个不同极性的组分；诱导分化3 T3-L1脂肪细胞，成熟后添加不同浓度的提取组分作用48 h，检测细胞培养液中葡萄糖、细胞白介素-6和游离脂肪酸含量，比较加药培养前后的葡萄糖、细胞白介素-6和游离脂肪酸含量，筛选出具有促进3 T3-L1脂肪细胞葡萄糖消耗、降低白细胞介素-6和游离脂肪酸分泌的活性组分。结果：胡柚果肉中极性组分占（88.96±2．34）％，而非极性组分的含量较少，胡柚果肉石油醚组分、二氯甲烷组分、乙酸乙酯组分和水组分均能显著促进脂肪细胞对葡萄糖的摄取，二氯甲烷组分、乙酸乙酯组分、正丁醇组分和水组分能够显著降低脂肪细胞白介素-6和游离脂肪酸的水平。结论：胡柚果肉可通过促进3 T3-L1脂肪细胞对葡萄糖的消耗，降低细胞因子白细胞介素-6和游离脂肪酸水平调节血糖。
目的：用不同極性的溶劑對鬍柚果肉進行依次分離，得到5箇不同極性組分，將不同極性組分進行促進3 T3-L1脂肪細胞葡萄糖消耗、降低白細胞介素-6和遊離脂肪痠分泌的活性組分的篩選及其機製的研究。方法：採用溶劑法將鬍柚果肉分離成5箇不同極性的組分；誘導分化3 T3-L1脂肪細胞，成熟後添加不同濃度的提取組分作用48 h，檢測細胞培養液中葡萄糖、細胞白介素-6和遊離脂肪痠含量，比較加藥培養前後的葡萄糖、細胞白介素-6和遊離脂肪痠含量，篩選齣具有促進3 T3-L1脂肪細胞葡萄糖消耗、降低白細胞介素-6和遊離脂肪痠分泌的活性組分。結果：鬍柚果肉中極性組分佔（88.96±2．34）％，而非極性組分的含量較少，鬍柚果肉石油醚組分、二氯甲烷組分、乙痠乙酯組分和水組分均能顯著促進脂肪細胞對葡萄糖的攝取，二氯甲烷組分、乙痠乙酯組分、正丁醇組分和水組分能夠顯著降低脂肪細胞白介素-6和遊離脂肪痠的水平。結論：鬍柚果肉可通過促進3 T3-L1脂肪細胞對葡萄糖的消耗，降低細胞因子白細胞介素-6和遊離脂肪痠水平調節血糖。
목적：용불동겁성적용제대호유과육진행의차분리，득도5개불동겁성조분，장불동겁성조분진행촉진3 T3-L1지방세포포도당소모、강저백세포개소-6화유리지방산분비적활성조분적사선급기궤제적연구。방법：채용용제법장호유과육분리성5개불동겁성적조분；유도분화3 T3-L1지방세포，성숙후첨가불동농도적제취조분작용48 h，검측세포배양액중포도당、세포백개소-6화유리지방산함량，비교가약배양전후적포도당、세포백개소-6화유리지방산함량，사선출구유촉진3 T3-L1지방세포포도당소모、강저백세포개소-6화유리지방산분비적활성조분。결과：호유과육중겁성조분점（88.96±2．34）％，이비겁성조분적함량교소，호유과육석유미조분、이록갑완조분、을산을지조분화수조분균능현저촉진지방세포대포도당적섭취，이록갑완조분、을산을지조분、정정순조분화수조분능구현저강저지방세포백개소-6화유리지방산적수평。결론：호유과육가통과촉진3 T3-L1지방세포대포도당적소모，강저세포인자백세포개소-6화유리지방산수평조절혈당。
Objective:To separate the Citrus changshan -huyou pulp with different polarities and to investigate the effects of flesh extracts from fruits in Citrus changshan -huyou Y.B.Chang on glucose uptake rate .To study IL-6 and FFA secretion in 3 T3-L1 and its mechanism .Methods:The Citrus changshan -huyou pulp was separated into five fractions with different polarities .The differentiation of 3 T3-L1 cells was induced ,then the adipocytes were treated with five frac-tions for 48 h ,and the glucose ,IL-6 and FFA in medium were detected , finally the fractions which could increase the glucose uptake and decrease IL -6 and FFA level in 3T3-L1 adipocytes were selected .Results:Polarity composition accounted for (88.96 ±2.34)%,and light petroleum fraction, methylene dichloride fraction, ethyl acetate fraction and water fraction could significantly increase the glucose uptake in 3 T3-L1 adipocytes;and the methylene dichloride frac-tion, ethyl acetate fraction , n-butyl alcohol fraction and water fraction could significantly decrease IL -6 and FFA lev-el in 3T3-L1 adipocytes.Conclusion:Pulp of Citrus changshan -huyou Y.B.Chang could adjust the blood sugar by promoting glucose uptake and decreasing IL -6 and FFA levels in 3 T3-L1 adipocytes .
