CAJ | 학술논문

目的：观察健脾益气中药对乙酸致胃溃疡大鼠胃组织中氨基己糖及前列腺素E2（PGE2）的影响，探讨健脾益气中药促进胃溃疡愈合的机制。方法：大鼠胃溃疡模型应用实验性乙酸法制备，随机分组，分别为空白对照组、模型组、健脾益气中药组和雷尼替丁组，连续给药治疗14天，采用酶联免疫法测定胃组织中PGE2的含量，用Morgan法原理测定胃组织氨基己糖。结果：与空白对照组大鼠比较，模型组大鼠胃组织PGE2含量明显降低（P ＜0.01），雷尼替丁组大鼠PGE2没有明显变化（P ＞0.05），健脾益气中药组大鼠 PGE2明显升高（P＜0.05）；雷尼替丁组大鼠和健脾益气中药组大鼠比模型组大鼠均明显升高（P ＜0.01）；健脾益气中药组大鼠胃组织PGE2明显高于雷尼替丁组大鼠（P ＜0.05）。与空白对照组大鼠比较，模型组大鼠和雷尼替丁组大鼠氨基己糖含量明显降低（P ＜0.01），健脾益气中药组大鼠没有明显变化（P ＞0.05）；模型组大鼠与雷尼替丁组大鼠差异无显著性（P ＞0.05），健脾益气中药组大鼠明显高于模型组大鼠（P ＜0.05）；与雷尼替丁组大鼠相比健脾益气中药组大鼠明显升高（P ＜0.05）。结论：健脾益气中药可以促进胃黏膜屏障的修复，使其防御功能接近正常水平，其机理可能是通过增加胃组织中氨基己糖、PGE2含量来完成的。
목적：관찰건비익기중약대을산치위궤양대서위조직중안기기당급전렬선소E2（PGE2）적영향，탐토건비익기중약촉진위궤양유합적궤제。방법：대서위궤양모형응용실험성을산법제비，수궤분조，분별위공백대조조、모형조、건비익기중약조화뢰니체정조，련속급약치료14천，채용매련면역법측정위조직중PGE2적함량，용Morgan법원리측정위조직안기기당。결과：여공백대조조대서비교，모형조대서위조직PGE2함량명현강저（P ＜0.01），뢰니체정조대서PGE2몰유명현변화（P ＞0.05），건비익기중약조대서 PGE2명현승고（P＜0.05）；뢰니체정조대서화건비익기중약조대서비모형조대서균명현승고（P ＜0.01）；건비익기중약조대서위조직PGE2명현고우뢰니체정조대서（P ＜0.05）。여공백대조조대서비교，모형조대서화뢰니체정조대서안기기당함량명현강저（P ＜0.01），건비익기중약조대서몰유명현변화（P ＞0.05）；모형조대서여뢰니체정조대서차이무현저성（P ＞0.05），건비익기중약조대서명현고우모형조대서（P ＜0.05）；여뢰니체정조대서상비건비익기중약조대서명현승고（P ＜0.05）。결론：건비익기중약가이촉진위점막병장적수복，사기방어공능접근정상수평，기궤리가능시통과증가위조직중안기기당、PGE2함량래완성적。
Objective:to observe the effect of Chinese drug of Jianpi Yiqi on aminohexose and Prostaglandin E2 in rats with acetic acid-induced stomach ulcer,and to explore the mechanism of the drug in improving the ulcer healing. Methods:Prepare the model rats with stomach ulcer by experimental acetic acid method,dividing them into control group,model group,drug group and ranitidine group,with successive administration for 14 days. Euzymelinked immunosorbent assay was taken to determine the PGE2 content,and Morgan to determine the aminohexose in stomach tissues. Results:Compared to control group,PGE2 was obviously decreased in model group (P <0.01);no obvious changes occurred in ranitidine group (P >0.05);PGE2 was obviously increased in drug group (P<0.05 );PGE2 in drug group was obviously higher than that in ranitidine group (P <0.05 ). Compared to control group,aminohex-ose in model group and ranitidine group significantly decreased (P <0.01 );no obvious changes occurred in drug group (P>0.05 );there was no significant difference between model group and ranitidine group (P >0.05 );drug group was significantly higher model group (P <0.05 );aminohexose in drug group was significantly higher than that in model group (P <0.05 ). Conclu-sion:Chinese drug of Jianpi Yiqi can promote the restoration of the stomach mucous membrane barrier and make its defense function approach to normal level,whose mechanism may be accomplished by increasing the aminohexose and PGE2.