CAJ | 학술논문

目的：建立HPLC法同时测定复方乳酸依沙吖啶溶液中乳酸依沙吖啶和盐酸苯海拉明的含量。方法：色谱柱为Luna氰基柱（4.6 mm×250 mm，5μm）；流动相为乙腈-水-三乙胺（50∶50∶0.5）；流速1.0 mL·min-1；柱温30℃；检测波长230 nm；进样量20μL。结果：乳酸依沙吖啶和盐酸苯海拉明与二苯酮分离良好。乳酸依沙吖啶在0.6012~1.4030 mg·mL-1范围内线性关系良好（r=0.9994），盐酸苯海拉明在0.1487~0.3469 mg·mL-1范围内线性关系良好（r=0.9999），依沙吖啶和苯海拉明的平均回收率分别为99.91%、99.73%，RSD分别为1.06%（n=9）、1.10%（n=9）。结论：本方法简便准确，快速可靠，可用于复方乳酸依沙吖啶溶液的含量测定和质量控制。
목적：건립HPLC법동시측정복방유산의사아정용액중유산의사아정화염산분해랍명적함량。방법：색보주위Luna청기주（4.6 mm×250 mm，5μm）；류동상위을정-수-삼을알（50∶50∶0.5）；류속1.0 mL·min-1；주온30℃；검측파장230 nm；진양량20μL。결과：유산의사아정화염산분해랍명여이분동분리량호。유산의사아정재0.6012~1.4030 mg·mL-1범위내선성관계량호（r=0.9994），염산분해랍명재0.1487~0.3469 mg·mL-1범위내선성관계량호（r=0.9999），의사아정화분해랍명적평균회수솔분별위99.91%、99.73%，RSD분별위1.06%（n=9）、1.10%（n=9）。결론：본방법간편준학，쾌속가고，가용우복방유산의사아정용액적함량측정화질량공제。
Objective: To establish the HPLC method for simultaneous determination of ethacridine lactate and benzhydramine hydrochloride in complex ethacridine lactate solution. Methods: The extract was separated by Luna CN column (4.6 mm × 250 mm, 5μm ), and the mobile phase was consisted of acetonitrile-water-triethylamine (50:50:0.5) with the lfow rate of 1.0 mL·min-1. The column temperature was 30℃. The detection wavelength was 230 nm and the injection volume was 20 μL. Results:The calibration curves of ethacridine lactate and benzhydramine hydrochloride were linear in the range of 0.601 2-1.403 0 mg·mL-1 (r = 0.999 4) and 0.148 7 - 0.346 9 mg· mL-1 (r = 0.999 9), respectively. The average recovery of ethacridine lactate was 99.91%with RSD of 1.06%(n=9). The average recovery of benzhydramine hydrochloride was 99.73%with RSD of 1.10%(n=9). Conclusion:The method is simple, accurate and reliable. It can be used as the quality control of complex ethacridine lactate solution.